Team:Groningen/protocols/GelElectrophoresis

From 2013.igem.org

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Line 26: Line 26:
<h5>Materials:</h5>
<h5>Materials:</h5>
<ul type="square">
<ul type="square">
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<li>Power supply</li>
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<li>TBE buffer 1x</li>
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<li>Gel tray</li>
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<li>TBE buffer 1x
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<li>0.8 or 1.5% agarose gel</li>
<li>0.8 or 1.5% agarose gel</li>
<li>2x Loading Dye
<li>2x Loading Dye

Revision as of 11:11, 28 July 2013

Gel electrophoresis

Materials:
  • TBE buffer 1x
  • 0.8 or 1.5% agarose gel
  • 2x Loading Dye
  • DNA samples
  • DNA 1kb GeneRuler of Fermentas

Procedure:
- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
- Load the samples on a 0.8% or 1.5% agarose gel
- Load the 1kb GeneRuler of Fermentas on gel
- Run the gel at 90V for 24-45 minutes to separate the bands.