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Team:Groningen/Labwork/20 June 2013
From 2013.igem.org
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<h2>Mirjam, Inne and Erik</h2> | <h2>Mirjam, Inne and Erik</h2> | ||
| - | + | Added 1 µl Serva/100 ml 1.5% agarose gel. | |
| - | <br | + | <br>Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water. |
| - | <br | + | <br>Made a 2x dilution of the loading buffer. |
| - | The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. | + | The <a href="protocols/Digestion"> restriction analysis <a/> of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. |
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The incubation is done at 37 degrees Celsius for 2 hours. | The incubation is done at 37 degrees Celsius for 2 hours. | ||
After incubation the restricted BBa_K844016, 2.5 ul 2x loading buffer + 3.5 ul restricted biobrick, is loaded on gel. | After incubation the restricted BBa_K844016, 2.5 ul 2x loading buffer + 3.5 ul restricted biobrick, is loaded on gel. | ||
| - | <br | + | <br>The gel electrophorese is done for 25 minutes at 90V. |
The picture is coming soon. | The picture is coming soon. | ||
Revision as of 14:14, 28 July 2013
Mirjam, Inne and Erik
Added 1 µl Serva/100 ml 1.5% agarose gel.Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water.
Made a 2x dilution of the loading buffer. The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. The incubation is done at 37 degrees Celsius for 2 hours. After incubation the restricted BBa_K844016, 2.5 ul 2x loading buffer + 3.5 ul restricted biobrick, is loaded on gel.
The gel electrophorese is done for 25 minutes at 90V. The picture is coming soon.
