Team:Groningen/Labwork/29 July 2013

(Difference between revisions)

Revision as of 08:12, 29 July 2013

Run the samples of the colony PCR of BBa_K823823 combined with the LacI promoter made on 27-07 on gel (order 1-8).
There are no bands around 1500 bp.

Run the purified PCR product of spec on gel.
The purified spec shows a nice band.

Run the PCR products of silk 2 on gel to do gel purification.
Only one of the tubes showed a band around the expected height.

Run the PCR products of silk 2 on gel to do gel purification.

Made a double restriction digestion with fast restriction digest for Pdes and CheY with respectively EcoRI and BamHI; and BamHI and PstI.

Made a ligation for the knockout system of Des, with Des UP, Tet and Des Down

Made a restriction digestion for Spec purified.

@@ Line 31: / Line 31: @@
 <p>Run the PCR products of silk 2 on gel to do gel purification.
 <br>Only one of the tubes showed a band around the expected height.
+<p>Run the PCR products of silk 2 on gel to do gel purification.
 <p>Made a double restriction digestion with fast restriction digest for Pdes and CheY with respectively EcoRI and BamHI; and BamHI and PstI.