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Team:DTU-Denmark/Notebook/29 July 2013
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| - | PCR programms based on [[Team:DTU-Denmark/Methods/PCR| standard PCR programm]] with differences in annealing temperatures and extension time as it was done on [ | + | PCR programms based on [[Team:DTU-Denmark/Methods/PCR| standard PCR programm]] with differences in annealing temperatures and extension time as it was done on [[Team:DTU-Denmark/Notebook/12_July_2013| 12-07-2013]. |
==Results== | ==Results== | ||
Revision as of 10:58, 29 July 2013
29 July 2013
Contents |
lab 208
Main purpose
- Colony PCR from Pseudomonas aeruginosa (PAO) to isolate Nir.
- Colony PCR to check the presence of AMO and HAO in transormants E.coli.
Who was in the lab
Kristian, Gosia, Henrike, Julia
Procedure
Colony PCR
Colony PCR was performed according to standard method in order to check if transformed cells which grew on medium contain desired insert in pZA21 plasmid. The colonies were chosen from agar plate to be checked. Names and numbers of colonies as well as plates names and primers are:
- HAO 1-6 in pZA21 USER, primers 18a, 18b
- AMO 1-9 in pZA21 USER, primers 17a, 17b
PCR programms based on standard PCR programm with differences in annealing temperatures and extension time as it was done on [[Team:DTU-Denmark/Notebook/12_July_2013| 12-07-2013].
Results
Conclusion
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