Team:Evry/Primers

From 2013.igem.org

(Difference between revisions)
Line 790: Line 790:
     </td>
     </td>
   </tr>
   </tr>
 +
   <tr>     
   <tr>     
     <td align="center">
     <td align="center">
Line 814: Line 815:
       <p>  
       <p>  
         CTGCAGGGTCTCACTAGTAGACGACTCCCTTTGATGAAC
         CTGCAGGGTCTCACTAGTAGACGACTCCCTTTGATGAAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P031
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°3 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGACAAATGCAAATAGTTATCCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P032
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°3 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGGATAACTATTTGCATTTGTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P033
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°4 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGACAAATAGTTATCAATAATCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P034
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°4 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGATTATTGATAACTATTTGTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P035
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°5 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGAGTAAATTAATATTATTTACAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P036
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°5 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGTAAATAATATTAATTTACTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P037
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°6 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGAAATAATGCTTCTCATTTTCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P038
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°6 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGAAAATGAGAAGCATTATTTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P039
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°7 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGAATAAATGATAATCATTATCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P040
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°7 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGATAATGATTATCATTTATTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P041
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°8 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGAGAAAATAATTCTTATTTCCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P042
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°8 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGGAAATAAGAATTATTTTCTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P043
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°9 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGAAGCACTTATTATTATTTTCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P044
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°9 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGAAAATAATAATAAGTGCTTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P045
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°10 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGAGATAATTGTTATCGTTTGCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P046
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°10 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGCAAACGATAACAATTATCTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P047
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°11 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGAGATAATGCTTATCAAAATCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P048
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°11 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGATTTTGATAAGCATTATCTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P049
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°12 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGACAAAATTATTATCACTTTCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P050
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°12 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGAAAGTGATAATAATTTTGTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P051
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°13 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGAAATAATGATTACCATTCCCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P052
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°13 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGGGAATGGTAATCATTATTTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P053
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°14 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGAGAAATTGTTTTTGATTTTCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P054
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°14 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGAAAATCAAAAACAATTTCTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P055
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°15 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGAGTTAATTGTAATGATTTTCAGATGCTAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P056
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Fur Binding Site N°15 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTAGCATCTGAAAATCATTACAATTAACTCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P057
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Andersen's promoter J23101 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GAATTCGGTCTCAGCCTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCGTGATGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P058
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Andersen's promoter J23101 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCATCACGCTAGCATAATACCTAGGACTGAGCTAGCTGTAAACTCTAGAGGCTGAGACCGAATTC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P059
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Andersen's promoter J23102 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACTGTGCTAGCGTGATGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P060
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Andersen's promoter J23102 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCATCACGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P061
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Andersen's promoter J23104 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCGTGATGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P062
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Andersen's promoter J23104 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCATCACGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P063
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        VR primer iGEM standard
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ATTACCGCCTTTGAGTGAGC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P064
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        VF2 primer iGEM standard
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        TGCCACCTGACGTCTAAGAA
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P065
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent A Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GAATTCGGTCTCTGTTCGGACCCATCTAGGTCCAGAGATAAGGAGGACAAACATGGATTTCAGCGGTAAAAATG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P066
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent A Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GGACTGCAGACTAGTGGTCTCTTGGATCTTATGCCCCCAGCGTTG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P067
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent B Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GAATTCGGTCTCTGTTCACATACTAAACACATCAATCAAGGAGGTACTAGGATGGCTATTCCAAAATTACAGGC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P068
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent B Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GGACTGCAGACTAGTGGTCTCTTGGATCTTATTTCACCTCGCGGG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P069
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent C Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GAATTCGGTCTCTTCCAATACTAAGCAGTTACAAGGATAAGGAGGAGCAACATGGATACGTCACTGGC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P070
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent C Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATGCAATCCAAAAACGTTCAAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P071
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent D Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GAATTCGGTCTCTTCCACACCCCGAAGAACAGCAAAGGCGGACGAACAATGGTCGATATGAAAACTACGC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P072
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent D Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATCGTGTTGGCACAGC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P073
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent E Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GAATTCGGTCTCTGAACGAGCTAAAAGTAGCGCCCAATATAAGGAGGTCAAAATGAGCATTCCATTCACCC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P074
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent E Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GGACTGCAGACTAGTGGTCTCTGTCTTCTCAGGCTGATGCGCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P075
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent F Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GAATTCGGTCTCTGAACACATCTATATTTATCTTAAGGAGTACAATCATGAGCCAGCATTTACCTTTG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P076
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Ent F Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GGACTGCAGACTAGTGGTCTCTGTCTTCTTACCTGTTTAGCGTTGCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P077
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        RBS sfGFP - Plasmid 3 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGTGAATTCGGTCTCTGTTCTGAAAGAACTATATCTACAAAATCCATATGCGTAAAGGCGAAGAG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P078
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        RBS sfGFP - Plasmid 3 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGTACAGTTCATCCATACCATG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P079
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Promoteur Lac + Lac Operator (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GAATTCGGTCTCAGCCTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCACAGAGTTCAGAGACCAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P080
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Promoteur Lac + Lac Operator (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GTGGTCTCTGAACTCTGTGCTCAGTATCTTGTTATCCGCTCACAATGTCAATTGTTATCCGCTCACAATTCTCTAGAGGCTGAGACCGAATTC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P081
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        RBS positive control - Plasmid 2 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        CGGTCTCTGTGATGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P082
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        RBS positive control - Plasmid 2 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCATCACAGAGACCG
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P083
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Lac I - Plasmid 1/2 (Forward - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GAATTCGGTCTCTCTAGATGGTGAATGTGAAACCAGTAAC
 +
      </p>
 +
    </td>
 +
  </tr>
 +
 +
  <tr>   
 +
    <td align="center">
 +
      <p>
 +
        P084
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        Lac I - Plasmid 1/2 (Reverse - GoldenBrick sequence)
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        --- bp
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        ---°C
 +
      </p>
 +
    </td>   
 +
    <td align="center">
 +
      <p>
 +
        GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTAAGCTACTAAAGCGTAGTTTTCG
       </p>
       </p>
     </td>
     </td>

Revision as of 16:52, 29 July 2013

Primers

CODE

NAME

LENGTH

ANNEALING
TEMPERATURE

SEQUENCE

P001

Primer Fur (Forward - GoldenBrick sequence)

--- bp

---°C

CGTGAATTCGGTCTCTCTAGATGACTGATAACAATACCGCC

P002

Primer Fur (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGCCTTCGTGCG

P005

RBS B0034 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTTGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA

P006

RBS B0034 (Reverse - GoldenBrick sequence)

--- bp

---°C

TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCAAGAGACCGAATTC

P007

aceB Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGATCTACGGCACATGAATCC

P008

ace B Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

TGCAGGGTCTCACTAGTACGTGCAGCTCCTCG

P009

Ent C Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGAAATCAGCTTCCTGTTATTAATAAGG

P010

Ent C Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

ACTAGTAATCATCCTCCACAAAATGATAAAGG

P011

Fec A Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGGGAAAATAATTCTTATTTCGATTGTCC

P012

Fec A Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTAACCTTCCCCATCATCATTTTTG

P013

Fec C Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGGAACAGCAATTCAACCTGC

P014

Fec C Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTATTCACAACGGTAAGCGG

P015

Andersen's promoter J23100 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCGTGATGAGACCG

P016

Andersen's promoter J23100 (Reverse - GoldenBrick sequence)

--- bp

---°C

CGGTCTCATCACGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAGGCTGAGACCGAATTC

P017

Fur Binding Site N°1 (Foward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAATTATTGATAACTATTTGGATGCTAGAGACCAC

P018

Fur Binding Site N°1 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCCAAATAGTTATCAATAATTCACAGAGACCG

P019

Fur Binding Site N°2 (Foward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGATAACTATTTGCATTTGCAGATGCTAGAGACCAC

P020

Fur Binding Site N°2 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGCAAATGCAAATAGTTATCTCACAGAGACCG

P021

Fep A Natural Fur promoter (Foward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGTTCGTCATTCAGACGCTG

P022

Fep A Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTATGTTTTATTCCTGCATTTTTGCC

P023

Fes Natural Fur promoter (Foward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGGCCACGAATTGCAACTG

P024

Fes Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTATTCGTCATTCAGACGCTG

P025

Sdh C Natural Fur promoter (Foward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGATCACTGTTCAGGATAAAACCC

P026

Sdh C Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTAGAATAACGCCCACATGC

P027

ybi L Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGCACCTAATTTGCCCATGATGATATCTC

P028

ybi L Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTAGTGATGGCTTCTGATAAAGATAAATTTATAG

P029

ync E Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGACGGGGGAAAGGACAAG

P030

ync E Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTAGACGACTCCCTTTGATGAAC

P031

Fur Binding Site N°3 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGACAAATGCAAATAGTTATCCAGATGCTAGAGACCAC

P032

Fur Binding Site N°3 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGGATAACTATTTGCATTTGTCACAGAGACCG

P033

Fur Binding Site N°4 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGACAAATAGTTATCAATAATCAGATGCTAGAGACCAC

P034

Fur Binding Site N°4 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGATTATTGATAACTATTTGTCACAGAGACCG

P035

Fur Binding Site N°5 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGTAAATTAATATTATTTACAGATGCTAGAGACCAC

P036

Fur Binding Site N°5 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGTAAATAATATTAATTTACTCACAGAGACCG

P037

Fur Binding Site N°6 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAAATAATGCTTCTCATTTTCAGATGCTAGAGACCAC

P038

Fur Binding Site N°6 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGAAAATGAGAAGCATTATTTCACAGAGACCG

P039

Fur Binding Site N°7 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAATAAATGATAATCATTATCAGATGCTAGAGACCAC

P040

Fur Binding Site N°7 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGATAATGATTATCATTTATTCACAGAGACCG

P041

Fur Binding Site N°8 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGAAAATAATTCTTATTTCCAGATGCTAGAGACCAC

P042

Fur Binding Site N°8 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGGAAATAAGAATTATTTTCTCACAGAGACCG

P043

Fur Binding Site N°9 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAAGCACTTATTATTATTTTCAGATGCTAGAGACCAC

P044

Fur Binding Site N°9 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGAAAATAATAATAAGTGCTTCACAGAGACCG

P045

Fur Binding Site N°10 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGATAATTGTTATCGTTTGCAGATGCTAGAGACCAC

P046

Fur Binding Site N°10 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGCAAACGATAACAATTATCTCACAGAGACCG

P047

Fur Binding Site N°11 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGATAATGCTTATCAAAATCAGATGCTAGAGACCAC

P048

Fur Binding Site N°11 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGATTTTGATAAGCATTATCTCACAGAGACCG

P049

Fur Binding Site N°12 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGACAAAATTATTATCACTTTCAGATGCTAGAGACCAC

P050

Fur Binding Site N°12 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGAAAGTGATAATAATTTTGTCACAGAGACCG

P051

Fur Binding Site N°13 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAAATAATGATTACCATTCCCAGATGCTAGAGACCAC

P052

Fur Binding Site N°13 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGGGAATGGTAATCATTATTTCACAGAGACCG

P053

Fur Binding Site N°14 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGAAATTGTTTTTGATTTTCAGATGCTAGAGACCAC

P054

Fur Binding Site N°14 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGAAAATCAAAAACAATTTCTCACAGAGACCG

P055

Fur Binding Site N°15 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGTTAATTGTAATGATTTTCAGATGCTAGAGACCAC

P056

Fur Binding Site N°15 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGAAAATCATTACAATTAACTCACAGAGACCG

P057

Andersen's promoter J23101 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCGTGATGAGACCG

P058

Andersen's promoter J23101 (Reverse - GoldenBrick sequence)

--- bp

---°C

CGGTCTCATCACGCTAGCATAATACCTAGGACTGAGCTAGCTGTAAACTCTAGAGGCTGAGACCGAATTC

P059

Andersen's promoter J23102 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACTGTGCTAGCGTGATGAGACCG

P060

Andersen's promoter J23102 (Reverse - GoldenBrick sequence)

--- bp

---°C

CGGTCTCATCACGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC

P061

Andersen's promoter J23104 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCGTGATGAGACCG

P062

Andersen's promoter J23104 (Reverse - GoldenBrick sequence)

--- bp

---°C

CGGTCTCATCACGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC

P063

VR primer iGEM standard

--- bp

---°C

ATTACCGCCTTTGAGTGAGC

P064

VF2 primer iGEM standard

--- bp

---°C

TGCCACCTGACGTCTAAGAA

P065

Ent A Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTGTTCGGACCCATCTAGGTCCAGAGATAAGGAGGACAAACATGGATTTCAGCGGTAAAAATG

P066

Ent A Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTTGGATCTTATGCCCCCAGCGTTG

P067

Ent B Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTGTTCACATACTAAACACATCAATCAAGGAGGTACTAGGATGGCTATTCCAAAATTACAGGC

P068

Ent B Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTTGGATCTTATTTCACCTCGCGGG

P069

Ent C Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTTCCAATACTAAGCAGTTACAAGGATAAGGAGGAGCAACATGGATACGTCACTGGC

P070

Ent C Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATGCAATCCAAAAACGTTCAAC

P071

Ent D Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTTCCACACCCCGAAGAACAGCAAAGGCGGACGAACAATGGTCGATATGAAAACTACGC

P072

Ent D Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATCGTGTTGGCACAGC

P073

Ent E Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTGAACGAGCTAAAAGTAGCGCCCAATATAAGGAGGTCAAAATGAGCATTCCATTCACCC

P074

Ent E Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTCAGGCTGATGCGCG

P075

Ent F Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTGAACACATCTATATTTATCTTAAGGAGTACAATCATGAGCCAGCATTTACCTTTG

P076

Ent F Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTACCTGTTTAGCGTTGCG

P077

RBS sfGFP - Plasmid 3 (Forward - GoldenBrick sequence)

--- bp

---°C

CGTGAATTCGGTCTCTGTTCTGAAAGAACTATATCTACAAAATCCATATGCGTAAAGGCGAAGAG

P078

RBS sfGFP - Plasmid 3 (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGTACAGTTCATCCATACCATG

P079

Promoteur Lac + Lac Operator (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCACAGAGTTCAGAGACCAC

P080

Promoteur Lac + Lac Operator (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTGAACTCTGTGCTCAGTATCTTGTTATCCGCTCACAATGTCAATTGTTATCCGCTCACAATTCTCTAGAGGCTGAGACCGAATTC

P081

RBS positive control - Plasmid 2 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGATGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA

P082

RBS positive control - Plasmid 2 (Reverse - GoldenBrick sequence)

--- bp

---°C

TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCATCACAGAGACCG

P083

Lac I - Plasmid 1/2 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTCTAGATGGTGAATGTGAAACCAGTAAC

P084

Lac I - Plasmid 1/2 (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTAAGCTACTAAAGCGTAGTTTTCG

Retrieved from "http://2013.igem.org/Team:Evry/Primers"