Team:Groningen/Labwork/30 July 2013

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(Difference between revisions)
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Started gel extraction for the restricted backbone+promoter, RFP and eYFP. RFP and eYFP are dissolved in 30µl elution buffer, to get a higher concentration
Started gel extraction for the restricted backbone+promoter, RFP and eYFP. RFP and eYFP are dissolved in 30µl elution buffer, to get a higher concentration
<br>Run a 0.8% agarose gel to examine the restriction.
<br>Run a 0.8% agarose gel to examine the restriction.
-
<br>Did a nanodrop on the samples to examine their concentration.
+
<br>Did a nanodrop on the samples to examine their concentration. Concentration of the backbone is 0.8 ng/&micro;l, RFP 6.4 ng/&micro;l and eYFP 2.4 ng/&micro;l.

Revision as of 07:04, 30 July 2013

Mirjam

Started gel extraction for the restricted backbone+promoter, RFP and eYFP. RFP and eYFP are dissolved in 30µl elution buffer, to get a higher concentration
Run a 0.8% agarose gel to examine the restriction.
Did a nanodrop on the samples to examine their concentration. Concentration of the backbone is 0.8 ng/µl, RFP 6.4 ng/µl and eYFP 2.4 ng/µl.