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Team:DTU-Denmark/Notebook/30 July 2013
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{{:Team:DTU-Denmark/Templates/StartPage|30 July 2013}} | {{:Team:DTU-Denmark/Templates/StartPage|30 July 2013}} | ||
| - | =lab | + | =lab 208= |
<hr/> | <hr/> | ||
| + | |||
==Main purpose== | ==Main purpose== | ||
<hr/> | <hr/> | ||
| + | Make PCR on Nir with genomic DNA template. | ||
| + | Amplify the pZE21 backbone with USER-primers for construction of the arabinose inducible system. | ||
| + | Put His-tag on the cycAX construct. | ||
==Who was in the lab== | ==Who was in the lab== | ||
<hr/> | <hr/> | ||
| + | Gosia, Kristian, Henrike, Julia | ||
==Procedure== | ==Procedure== | ||
<hr/> | <hr/> | ||
| + | |||
| + | ===PCR=== | ||
| + | *pZE21::GFP SF and pZE21::RFP in duplicates with primers pair 13 on 55C and extension time 3:00. | ||
| + | *cycAX in triplicates with primer pair 33 2 samples on 62C and 3:30 extension and 1 sample on 55C and extension 3:00. | ||
| + | *None negative was made for each containing same things as real samples just MQ instead of template DNA. | ||
| + | |||
==Results== | ==Results== | ||
<hr/> | <hr/> | ||
| + | ===Gel on PCR from yesterday=== | ||
| + | *1kb ladder | ||
| + | *Nir 1uL genomic template | ||
| + | *Nir 3uL genomic template | ||
| + | *Nir 7uL.... | ||
| + | *Nir 10uL.... | ||
| + | *Nir 12uL.... | ||
| + | *Nir 15uL.... | ||
| + | *Nir 1uL HindIII treated genomic template | ||
| + | *Nir 3uL HindIII treated genomic template | ||
| + | *Nir 7uL.... | ||
| + | *Nir 10uL.... | ||
| + | *Nir 12uL.... | ||
| + | *Nir 15uL.... | ||
| + | *Negative control | ||
| + | *1kb ladder | ||
| + | [[File:2013 07 30 gel nir genomic dna.jpg|600px]] | ||
==Conclusion== | ==Conclusion== | ||
<hr/> | <hr/> | ||
| + | No right bands where acquired from PCR-amplification with genomic DNA template. | ||
| + | |||
Navigate to the [[Team:DTU-Denmark/Notebook/29_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/31_July_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/29_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/31_July_2013|Next]] Entry | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Revision as of 12:10, 30 July 2013
30 July 2013
Contents |
lab 208
Main purpose
Make PCR on Nir with genomic DNA template. Amplify the pZE21 backbone with USER-primers for construction of the arabinose inducible system. Put His-tag on the cycAX construct.
Who was in the lab
Gosia, Kristian, Henrike, Julia
Procedure
PCR
- pZE21::GFP SF and pZE21::RFP in duplicates with primers pair 13 on 55C and extension time 3:00.
- cycAX in triplicates with primer pair 33 2 samples on 62C and 3:30 extension and 1 sample on 55C and extension 3:00.
- None negative was made for each containing same things as real samples just MQ instead of template DNA.
Results
Gel on PCR from yesterday
- 1kb ladder
- Nir 1uL genomic template
- Nir 3uL genomic template
- Nir 7uL....
- Nir 10uL....
- Nir 12uL....
- Nir 15uL....
- Nir 1uL HindIII treated genomic template
- Nir 3uL HindIII treated genomic template
- Nir 7uL....
- Nir 10uL....
- Nir 12uL....
- Nir 15uL....
- Negative control
- 1kb ladder
Conclusion
No right bands where acquired from PCR-amplification with genomic DNA template.
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