Team:UTK-Knoxville/5 June 2013

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<a href="../UTK-knoxville/2013.igem.org">2013.igem.org</a></p>
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<div id="header"><a href="https://2013.igem.org"><img src="https://static.igem.org/mediawiki/2013/thumb/2/20/Header2.jpg/800px-Header2.jpg" alt="Team UTK-Knoxville" width="910" height="234"></a></div>
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overview</span></span></a></li>
 
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a>
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</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION1</span></span></a></li>
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</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/partsorigin"><span><span>Parts Origin</span></span></a></li>
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/construct"><span><span>Construct</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION3</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION3</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li>
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                               <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Project Overview</span></span></a></li>
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      <li><a href="https://2013.igem.org/Team:UTK-Knoxville/EastmanHITES"><span><span>Eastman HITES</span></span></a></li>
                               <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section2</span></span></a></li>
                               <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section2</span></span></a></li>
                               <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section3</span></span></a></li>
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<a href="https://2013.igem.org/Team:UTK-Knoxville/sponsors" style="color: white">Sponsors</a>
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<h1><span class="mw-headline">June 5th 2013</span></h1>
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<p>Today we isolated OmpR again.  We used the POmpR_F and POmpR_R primers.  Testing 60 C and 64 C.  This time we used the Protocol for Q5® High-Fidelity 2X Master Mix on NEB’s website, as opposed to the slightly modified phusion protocol we have been using.</p>
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<h3> <span class="mw-headline" id="Wednesday_05_June_013"> Wednesday 05 June 13 </span>     </h3>
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<li> Today we isolated OmpR again.  We used the POmpR_F and POmpR_R primers.  Testing 60°C and 64°C.  This time we used the Protocol for Q5® High-Fidelity 2X Master Mix on NEB’s website.</li>
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Latest revision as of 20:02, 30 July 2013

Team:UK-Knoxville - 2013.igem.org

NotebookNAVI NotebookRETURN BACK NEXT

June 5th 2013

Today we isolated OmpR again. We used the POmpR_F and POmpR_R primers. Testing 60 C and 64 C. This time we used the Protocol for Q5® High-Fidelity 2X Master Mix on NEB’s website, as opposed to the slightly modified phusion protocol we have been using.

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UTK.edu UTK CBE UTK CBE Trinh Lab