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Team:Evry/Protocols/02
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| - | <h1 align='center'> | + | <h1 align='center'> Protocol for transformation </h1> |
<p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA.<br/> | <p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA.<br/> | ||
Revision as of 11:08, 31 July 2013
Protocol for transformation
For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA.
Let 30 minutes on ice.
Let the cells at 42°C for exactly 50 seconds.
Let 5 minutes on ice.
Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.
