"
Team:Evry/Protocols/02
From 2013.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
<h1 align='center'> Protocol for transformation </h1> | <h1 align='center'> Protocol for transformation </h1> | ||
| - | <p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA.<br/> | + | <p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. IF the ligation protocol is a golden gate, add 5 µL instead.<br/> |
Let 30 minutes on ice.<br/> | Let 30 minutes on ice.<br/> | ||
Let the cells at 42°C for exactly 50 seconds.<br/> | Let the cells at 42°C for exactly 50 seconds.<br/> | ||
Revision as of 14:10, 1 August 2013
Protocol for transformation
For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. IF the ligation protocol is a golden gate, add 5 µL instead.
Let 30 minutes on ice.
Let the cells at 42°C for exactly 50 seconds.
Let 5 minutes on ice.
Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.
