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| - | <html>
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| - | <head>
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| - | <style type="text/css">
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| - | .firstHeading { /* Gets rid of the default iGEM picture*/
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| - | height:2px;
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| - | visibility:hidden;
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| - | }
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| - |
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| - | #contentSub, #search-controls, .firstHeading, #catlinks, #p-logo, .editsection {
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| - | display:none;
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| - | }
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| - |
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| - | #content {
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| - | background-color: transparent;
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| - | border-left-width:0px;
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| - | border-right-width:0px;
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| - | padding: 0px 5px 0px 5px;
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| - | }
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| - |
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| - | #footer-box {
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| - | background:none;
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| - | border:none;
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| - | }
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| - |
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| - | #menubar {
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| - | display: table-cell;
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| - | background: transparent;
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| - | margin-top:90px;
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| - | margin-left: 200px;
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| - | margin-right: 230px;
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| - | }
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| - | #menubar * {
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| - | color: white !important;
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| - | background: white !important;
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| - | }
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| - |
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| - | #menubar:hover * {
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| - | color: white !important;
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| - | }
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| - | #p-logo {
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| - | overflow:hidden;
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| - | display: none;
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| - | }
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| - |
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| - | #top-section {
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| - | heigth:0px;
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| - | width: 100%;
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| - | margin:0px;
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| - | padding: 0px;
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| - | background-repeat: no-repeat;
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| - | border-width:0px;
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| - | border-top-width:0px;
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| - | }
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| - | #toc{
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| - | visibility:hidden;
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| - | display:none;
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| - | }
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| - |
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| - | #siteSub {
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| - | display:none;
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| - | }
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| - | #search-controls {
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| - | display:none;
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| - | margin-top:0px;
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| - | }
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| - |
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| - | #contentSub {
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| - | display:none;
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| - | }
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| - |
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| - | /*Now to set heading and body fonts*/
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| - | body {
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| - | width:1000px;
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| - | /* background: url("Hintergrund_linear.gif") center top;*/
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| - | background-color: #2E2E2E;
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| - |
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| - | background-width: 100%;
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| - | margin-top:0px; /*-80px*/
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| - | float:center;
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| - | postion: absolute;
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| - | }
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| - | #menu {
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| - | width:100%;
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| - | height: 3em;
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| - | float: left;
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| - | position: abolute;
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| - | margin-top:150px;
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| - | background-color: transparent;
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| - | background-repeat: no-repeat;
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| - | border-bottom: 8px #FFBF00 solid;
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| - | }
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| - | #menu ul li {
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| - | background-color:transparent;
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| - | list-style: none;
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| - | float: left;
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| - | }
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| - | #menu ul li a {
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| - | background-color: #2E2E2E;
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| - | padding-bottom: 5px;
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| - | padding-right: 20px;
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| - | padding-top: 5px;
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| - | display: block;
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| - |
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| - | color:white ;
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| - | text-decoration: none;
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| - | font-weight:bold;
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| - | font-family: Arial;
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| - | font: 1.2em/1.5em;
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| - |
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| - | /*text-shadow:#FFBF00 1px 2px;*/
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| - | letter-spacing:0.1em;
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| - | }
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| - | #menu a:hover {
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| - | background: #2E2E2E;
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| - | color:#FFBF00;
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| - | }
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| - | #menu li {
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| - | position: relative;
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| - | float: center;
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| - | }
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| - |
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| - | #navbar ul {
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| - | position: absolute;
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| - | z-index: 500;
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| - | }
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| - |
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| - | #navbar ul ul {
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| - | position: absolute;
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| - | top: 10px;
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| - | left: 50%;
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| - | }
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| - |
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| - | #navbar ul,
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| - | #navbar li:hover ul ul,
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| - | #navbar ul li:hover ul ul{
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| - | display: none;
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| - | }
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| - |
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| - | #navbar li:hover ul,
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| - | #navbar ul li:hover ul,
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| - | #navbar ul ul li:hover ul,
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| - | #navbar li:hover img {
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| - | display: block;
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| - | visibility:visible;
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| - | margin: 0;
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| - | padding: 0;
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| - | }
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| - |
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| - | #logo_off{
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| - | z-index:2;
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| - | position:absolute;
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| - | float: right;
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| - | width:7%;
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| - | margin-right:0px;
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| - | margin-top:0px;
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| - | margin-left:900px;
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| - | }
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| - |
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| - | #logo {
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| - | z-index:1;
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| - | position:absolute;
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| - | }
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| - | #logo img{
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| - |
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| - | float:left;
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| - | width:1000px;
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| - | }
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| - | #left {
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| - | padding-left:20px;
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| - | padding-top: 50px;
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| - | width:50%;
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| - | float:left;
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| - | position:relative;
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| - | color:white;
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| - | font-family: Arial;
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| - | text-align:justify;
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| - | }
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| - |
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| - | #right {
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| - | padding-top: 100px;
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| - | width:40%;
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| - | float:right;
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| - | position:relative;
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| - | }
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| - | #h1 {
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| - |
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| - | font-color:white;
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| - | }
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| - |
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| - | </style>
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| - |
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| - | <script>
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| - | sfHover = function()
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| - | {
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| - | var sfEls = document.getElementById("navbar").getElementsByTagName("li");
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| - | for (var i=0; i<sfEls.length; i++)
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| - | {
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| - | sfEls[i].onmouseover=function()
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| - | {
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| - | this.className+=" hover";
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| - | }
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| - | sfEls[i].onmouseout=function()
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| - | {
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| - | this.className=this.className.replace(new RegExp(" hover\\b"), "");
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| - | }
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| - | }
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| - | }
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| - | if (window.attachEvent) window.attachEvent("onload", sfHover);
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| - | </script>
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| - |
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| - | </head>
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| - |
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| - | <body>
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| - |
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| - | <div id="header">
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| - | <div id="logo" style="z-index: 8;"><img src="Logo.png"/></div>
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| - | <div id="logo_off" style="z-index: 10;"><a href="https://2013.igem.org/Main_Page"><img src="IGEM_Logo.gif" width=100%/></a></div>
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| - |
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| - | </div>
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| - |
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| - | <div id="menu">
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| - | <ul id="navbar">
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| - | <li><a href="https://2013.igem.org/Team:Freiburg">Home</a>
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Team">Team</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Team2">Members</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/gallery">Photo Gallery</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/Coop">Collaborations</a></li>
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| - | </ul>
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project">Project</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/1">1</a>
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| - | <!-- <ul>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/1/Labbook">Labbook</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/1/Results">Results</a></li>
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| - | </ul> -->
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/2">2</a>
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| - | <!-- <ul>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/2/Labbook">Labbook</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/2/Results">Results</a></li>
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| - | </ul> -->
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/3">3</a>
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| - | <!-- <ul>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/3/Labbook">Labbook</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/3/Results">Results</a></li>
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| - | </ul> -->
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/4">4</a>
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| - | <!-- <ul>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/4/Labbook">Labbook</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/4/Results">Results</a></li>
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| - | </ul> -->
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/5">5</a>
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| - | <!-- <ul>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/5/Labbook">Labbook</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/5/Results">Results</a></li>
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| - | </ul> -->
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/6">6</a>
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| - | <!-- <ul>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/6/Labbook">Labbook</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Project/6/Results">Results</a></li>
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| - | </ul> -->
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| - | </li>
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| - | </ul>
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/HumanPractice">Human Practice</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/HumanPractice/Facebook">Facebook</a></li>
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| - | </ul>
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Modeling">Modeling</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Modeling/crRNADesign">crRNADesign</a></li>
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| - | </ul>
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Notebook">Notebook</a>
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Safety">Safety</a>
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| - | </li>
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| - | <li><a href="https://2013.igem.org/Team:Freiburg/Attributions">Attributions</a>
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| - | </li>
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| - | </ul>
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| - | </div>
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| - | <div id="left">
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| - | <h1 id="h1">Project Description</h1>
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| - | <p><font size="5">I</font>f one fact is true: Biology proved itself to be more complex than we could ever have imagined. This especially holds true for fields like cancer research and cell reprogramming.
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| - | Up to several years ago the main approach for investigating gene signaling cascades was to focus on single genes and underestimating crosstalk in between pathways. Investigating on those crosstalks was a difficult, time consuming work, that is up to date a challenge for scientists all around the world. High throughput methods like whole transcriptome analysis gave rise to complete new approaches and ideas. One major flaw of these methods is, that they are only descriptive and do not allow to interfere with those systems.</p>
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| - | <p>Here, we demonstrate a system, based on a protein-RNA-DNA interaction that allows multiple gene regulation simultaneously with minimal effort. Cas9, a nuclease, derived from a bacterial immunity system, is able to bind to multiple RNA specified targets.
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| - | The FreiGEM 2013 team aims to develop from this protein a new class of DNA binding proteins by mutating the nuclease and by fusing it to effector domains. The aim was not only to modify gene expression, but also to make this process inducible in a spatiotemporal manner. This gives rise to application in medical and fundamental research.</p>
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| - | <p>For a proof-of-principle work we demonstrate function in human cell lines and evaluated those results by theoretical modeling.
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| - | Furthermore, we were able to manipulate endogenous targets in the well-studied model organisms Danio rerio and Arabidopsis thaliana, by targeting factors involved into stem cell reprogramming stem cells were induced and endogenous reporters, respectively.</p>
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| - | </div>
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| - | <div id="right">
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| - | <img src="CRISPR.gif" width="400px">
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| - | </div>
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| - | </body>
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| - | </html>
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