"
Team:DTU-Denmark/Notebook/3 August 2013
From 2013.igem.org
(→Miniprep) |
|||
| Line 8: | Line 8: | ||
==Who was in the lab== | ==Who was in the lab== | ||
<hr/> | <hr/> | ||
| + | Henrike, Kristian | ||
==Procedure== | ==Procedure== | ||
| Line 20: | Line 21: | ||
Isolate plasmids from samples Sec2, cycAX, TAT 3-1a, TAT 3-2, TAT 2-1 using [[Team:DTU-Denmark/Methods/Plasmid_isolation|ethanol precipitation]]. Actually should have used the Midiprep kit from QIAgen. | Isolate plasmids from samples Sec2, cycAX, TAT 3-1a, TAT 3-2, TAT 2-1 using [[Team:DTU-Denmark/Methods/Plasmid_isolation|ethanol precipitation]]. Actually should have used the Midiprep kit from QIAgen. | ||
After ethanol precipitation continued with Midiprep kit step from step 9. | After ethanol precipitation continued with Midiprep kit step from step 9. | ||
| + | |||
| + | ===Purification gel=== | ||
| + | Purification of Nir 2 fragment by gel purification. | ||
| + | |||
==Results== | ==Results== | ||
| Line 48: | Line 53: | ||
==Conclusion== | ==Conclusion== | ||
<hr/> | <hr/> | ||
| + | The gel verified that Nir1 high is the right fragment but also confirmed that the recurrent fragment on approx. 16 kb from whole Nir isolation is not Nir but unspecific product. | ||
| + | |||
Navigate to the [[Team:DTU-Denmark/Notebook/2_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/4_August_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/2_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/4_August_2013|Next]] Entry | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Revision as of 14:57, 4 August 2013
3 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Henrike, Kristian
Procedure
Gel electrophoresis
Run 1% agarose gel on overnight PCR products.
Miniprep
Isolate plasmids from samples Sec2, cycAX, TAT 3-1a, TAT 3-2, TAT 2-1 using ethanol precipitation. Actually should have used the Midiprep kit from QIAgen. After ethanol precipitation continued with Midiprep kit step from step 9.
Purification gel
Purification of Nir 2 fragment by gel purification.
Results
Gels
1% agarose gel
- 1kb ladder
- Nir1 high
- Nir1 high
- Nir1 low
- Nir1 low
- broad band ladder
- 43
- 43
- Nir2 GC
- Nir2 GC
- neg for Nir
- AMO
- AMO
- neg for AMO
- 1 kb ladder
Conclusion
The gel verified that Nir1 high is the right fragment but also confirmed that the recurrent fragment on approx. 16 kb from whole Nir isolation is not Nir but unspecific product.
Navigate to the Previous or the Next Entry
