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Revision as of 22:57, 6 August 2013 (view source) Sgrenz (Talk | contribs) ← Older edit		Revision as of 22:58, 6 August 2013 (view source) Sgrenz (Talk | contribs) m Newer edit →
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	<h1>PCR Protocols and Programs</h1>		<h1>PCR Protocols and Programs</h1>
-	<h3>Phusion 25 µl</h3>	+	<h3><a name="Phusion">Phusion 25 µl</h3>
	<table border=1 style="float:left">		<table border=1 style="float:left">

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Revision as of 22:58, 6 August 2013

PCR Protocols and Programs

Phusion 25 µl

Reagenz	1x	8x
Buffer 5x	5,0	44
MgSO4	0	0
dNTPs	2,5	22
Primer1	1,0	8,8
Primer2	1,0	8,8
Template	1,5	13,2
DMSO	2,0	17,6
Polymerase	0,5	4,4
H2O	11,5	101,2

Program Phusion
98 °C	98 °C	x °C	72 °C	72 °C
2 min	30 s	30 s	y	6 min
x: according to Primer Tm y: 15s/kb