Team:Groningen/Labwork/7 August 2013

From 2013.igem.org

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<br>Made a restriction digestion of CheY with BamHI and SpeI.
<br>Made a restriction digestion of CheY with BamHI and SpeI.
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<h2>Chaline</h2>
<h2>Chaline</h2>
Measured the concentration of three mini prep tubes of CFP and one of the restriction digests.
Measured the concentration of three mini prep tubes of CFP and one of the restriction digests.

Revision as of 09:52, 7 August 2013

Mirjam

A check on the plates with CFP revealed that no colonies are present.
A check on the plates for ΔCheY revealed colonies. The ΔDes plates did not reveal any colonies.
Check of the restriction digest made 06-08 of CheY, and the two tubes of CFP. This reveals that CheY and one of the CFP tubes is totally absent.
Made a restriction digestion of GFP/YFP with Xba and PstI and did gel purification.
Made a restriction digestion of CFP with EcoRI and PstI and did gel purification.
Did a gel purication on BBa_k823823 restricted with EcoRI and SpeI.
Made a restriction digestion of CheY with BamHI and SpeI.

Chaline

Measured the concentration of three mini prep tubes of CFP and one of the restriction digests.
Measured the concentration of the restricted GFP, YFP and BBa_k823823.