Team:Groningen/Labwork/8 August 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "<html> <head> <meta http-equiv="X-UA-Compatible" content="IE=EDGE" charset="utf-8" /> <link rel="stylesheet" href="https://2013.igem.org/Team:Groningen/CSS/DefaultPage?action=ra...")
Line 26: Line 26:
<br>Inoculated the colony of CFP and four colonies of Pdes/CheY/eYFP in LB medium with amp for mini prep.  
<br>Inoculated the colony of CFP and four colonies of Pdes/CheY/eYFP in LB medium with amp for mini prep.  
<br>Made a new ligation reaction between Pdes and CheY.  
<br>Made a new ligation reaction between Pdes and CheY.  
 +
<br>Made a new ligation reaction between CFP and the backbone.
 +
<br>Made a new ligation reaction between Pdes/CheY and the backbone.
 +
<br>Transformation to <i>E. coli</i> of all the ligation reactions.
 +
</div>
</div>

Revision as of 10:11, 8 August 2013

Mirjam

Check of the plates of the restrictions made yesterday. One of the plates with CFP showed 1 colony. The other two plates (one IPTG) did not show any. The transformation of Pdes/CheY into the backbone failed, but the transformation of Pdes/CheY/eYFP showed a couple of colonies.
Inoculated the colony of CFP and four colonies of Pdes/CheY/eYFP in LB medium with amp for mini prep.
Made a new ligation reaction between Pdes and CheY.
Made a new ligation reaction between CFP and the backbone.
Made a new ligation reaction between Pdes/CheY and the backbone.
Transformation to E. coli of all the ligation reactions.