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Team:Grenoble-EMSE-LSU
From 2013.igem.org
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<img src="https://static.igem.org/mediawiki/2013/9/9a/1ere_version_image.png" alt="Project overview" width="600px" /> | <img src="https://static.igem.org/mediawiki/2013/9/9a/1ere_version_image.png" alt="Project overview" width="600px" /> | ||
| - | <figcaption>Overview on the <em>Light Automated Cell Control</em> (Lac²) Project</figcaption> | + | <!--<figcaption>Overview on the <em>Light Automated Cell Control</em> (Lac²) Project</figcaption>--> |
</figure> | </figure> | ||
Our system involves Escherichia Coli (E. coli) bacteria, producing the photosensitizing protein KillerRed. Upon light irradiation, this fluorescent protein (580/630 nm) produces Reactive Oxygen Species (ROS) which irreversibly damage cell proteins, membranes and DNA, leading to cell death [2]. Bacterial growth is here followed by monitoring the KillerRed-expressing cell red fluorescence and can be controlled by modulating the amount of ROS produced inside the bacteria, using white light stimulations. Since the amount of ROS produced, and thus the cytotoxicity, is closely related to the concentration in intracellular KillerRed, a photosensitive expression system enabling to control this protein level inside the cell was also developed.<br><br> | Our system involves Escherichia Coli (E. coli) bacteria, producing the photosensitizing protein KillerRed. Upon light irradiation, this fluorescent protein (580/630 nm) produces Reactive Oxygen Species (ROS) which irreversibly damage cell proteins, membranes and DNA, leading to cell death [2]. Bacterial growth is here followed by monitoring the KillerRed-expressing cell red fluorescence and can be controlled by modulating the amount of ROS produced inside the bacteria, using white light stimulations. Since the amount of ROS produced, and thus the cytotoxicity, is closely related to the concentration in intracellular KillerRed, a photosensitive expression system enabling to control this protein level inside the cell was also developed.<br><br> | ||
Revision as of 21:16, 8 August 2013
