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09/08/13
From 2013.igem.org
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*Pellet resuspended in 375ul of HMFM | *Pellet resuspended in 375ul of HMFM | ||
*samples were then frozen at -80 | *samples were then frozen at -80 | ||
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| + | ==Restriction mapping== | ||
Revision as of 14:35, 9 August 2013
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Isolating plasmid
- From overnight culture, took 3ml of culture and centrifuged into pellet of samples 5.1, 10.1, 10.2, 10.3
- Isolated the plasmid using omega bio-tek Plasmid mini kit I
- Concentrations from nano drop are shown in the table below
| Sample | Volume(ul) | Conc.(ng/ul) | 260/280 | 260/230 |
| 5.1 | 92 | 65.8 | 1.87 | 1.75 |
| 10.1 | 88 | 36.9 | 1.80 | 1.77 |
| 10.2 | 89 | 48.3 | 1.79 | 1.98 |
| 10.3 | 94 | 17.4 | 1.84 | 1.59 |
Glycerol stocks
- Took 750ul from overnight stock and centrifuged
- Supernatant was removed
- Pellet resuspended in 375ul of HMFM
- samples were then frozen at -80
