Revision as of 13:31, 11 August 2013

Notebook : August 9

Abdou, Anais, Damir, Nadia, XiaoJing

We do the exprience again because the lysis made on wensday didn't happen. It's probably because the phage strain was too old ( 2001)

Picture: lysed cells comparison.

0µl phage(control): the petri dish is cloudy, bacteria are not lysed.
50µl phage: the petri dish is clear, bacteria are lysed by phages.
We used a wild type strain to keep a stock and a Δ fnr E. coli strain to obtain phages that might have encapsidated a Δfnr::Km fragment.

10µl,50µl and 100µl petri dishes are clear so phages are multiplied.

We let the antibiotic over night to select the right strain.

Abdou

Clone 10,14 and 15 plamid extraction using nucleospin kit.

Results: concentration measured by nanodrop

We still have to sequence plasmids in order to verify our results.

Carry out

@@ Line 29: / Line 29: @@
 ===='''2 - Obtaining RBS_lacZ_ter. '''====
+Abdou
 Clone 10,14 and 15 plamid extraction using nucleospin kit.
@@ Line 39: / Line 40: @@
 *clone 15: C=52 ng/µl     260/280=1.78
 We still have to sequence plasmids in order to verify our results.
+==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''===
+Carry out
 {{Team:Paris_Saclay/incl_fin}}