Team:Glendale CC AZ/Protocols/SurivalGrowth

From 2013.igem.org

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== Survival Growth Assay ==
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== Materials ==
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    -P200 micropipette
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    -10 microliters bacteria
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    -90 mictroliters LB media
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    -Agar plates with appropriate antibiotic
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    -Spreader
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    -20mL ethanol (to sterilize spreader)
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    -Incubator set to 37 degrees celcius.
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== Procedure ==
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1. Label all plates (Keep upside down to avoid condensation on agar)
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2. Pipette LB media into tube containing bacteria; aspirate.
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3. Using P200 micropipette, extract all 100 microliters of solution and place onto appropriate agar plate.
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4. Briefly flame the spreader and let cool momentarily.
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5. Spread bacteria-LB solution all over plate.
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6. Incubate at 37 degrees celcius for up to 24 hours.

Revision as of 18:58, 11 August 2013


Survival Growth Assay

Materials 

   -P200 micropipette
   -10 microliters bacteria
   -90 mictroliters LB media
   -Agar plates with appropriate antibiotic
   -Spreader
   -20mL ethanol (to sterilize spreader)
   -Incubator set to 37 degrees celcius.


Procedure

1. Label all plates (Keep upside down to avoid condensation on agar) 2. Pipette LB media into tube containing bacteria; aspirate. 3. Using P200 micropipette, extract all 100 microliters of solution and place onto appropriate agar plate. 4. Briefly flame the spreader and let cool momentarily. 5. Spread bacteria-LB solution all over plate. 6. Incubate at 37 degrees celcius for up to 24 hours.

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