"
Team:Glendale CC AZ/Protocols/SurivalGrowth
From 2013.igem.org
(Difference between revisions)
E.Soboslay (Talk | contribs) |
E.Soboslay (Talk | contribs) |
||
| Line 13: | Line 13: | ||
-Spreader | -Spreader | ||
-20mL ethanol (to sterilize spreader) | -20mL ethanol (to sterilize spreader) | ||
| - | -Incubator set to | + | -Incubator set to 37°C. |
| Line 28: | Line 28: | ||
5. Spread bacteria-LB solution all over plate. | 5. Spread bacteria-LB solution all over plate. | ||
| - | 6. Incubate at | + | 6. Incubate at 37°C for up to 24 hours. |
Revision as of 22:55, 11 August 2013
Survival Growth Assay
Materials
-P200 micropipette -10µl bacteria -90µl LB media -Agar plates with appropriate antibiotic -Spreader -20mL ethanol (to sterilize spreader) -Incubator set to 37°C.
Procedure
1. Label all plates (Keep upside down to avoid condensation on agar)
2. Pipette LB media into tube containing bacteria; aspirate.
3. Using P200 micropipette, extract all 100µl of solution and place onto appropriate agar plate.
4. Briefly flame the spreader and let cool momentarily.
5. Spread bacteria-LB solution all over plate.
6. Incubate at 37°C for up to 24 hours.
