Team:Ciencias-UNAM

From 2013.igem.org

(Difference between revisions)
Line 1: Line 1:
-
 
<html>
<html>
-
<head>
 
-
 
-
<title>Home</title>
 
-
<meta http-equiv="content-type" content="text/html; charset=utf-8"/>
 
-
<meta http-equiv="imagetoolbar" content="no"/>
 
-
<meta name="apple-mobile-web-app-capable" content="yes"/>
 
-
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:Ciencias-UNAM/stylesheets/rp_page?action=raw&ctype=text/css"/>
 
-
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:Ciencias-UNAM/stylesheets/specificstyle_Home?action=raw&ctype=text/css"/>
 
-
<script src="https://2013.igem.org/Team:Ciencias-UNAM/scripts/sitemap?action=raw&ctype=text/javascript"></script>
 
-
<script src="https://2013.igem.org/Team:Ciencias-UNAM/scripts/jquery?action=raw&ctype=text/javascript"></script>
 
-
<script src="https://2013.igem.org/Team:Ciencias-UNAM/scripts/axutils?action=raw&ctype=text/javascript"></script>
 
-
<script src="https://2013.igem.org/Team:Ciencias-UNAM/scripts/jquery1.8?action=raw&ctype=text/javascript"></script>
 
-
<script src="https://2013.igem.org/Team:Ciencias-UNAM/scripts/beforepagescript?action=raw&ctype=text/javascript"></script>
 
-
<script src="https://2013.igem.org/Team:Ciencias-UNAM/scripts/messagecenter?action=raw&ctype=text/javascript"></script>
 
-
<script src='https://2013.igem.org/Team:Ciencias-UNAM/scripts/data_Home?action=raw&ctype=text/javascript'></script>
 
-
 
-
</head>
 
-
 
<body>
<body>
 +
<img src="https://static.igem.org/mediawiki/2013/0/0a/U42_normal.png" class="raw_image">
-
<div id="main_container">
+
<b>Abstract</b>  
-
 
+
<p>The human peptide LL-37 is a cationic antimicrobial peptide with activity against Gram-negative and Gram-positive bacteria. It has been shown to protect against <i>Helicobacter pylori</i> infection and its related gastritis, besides of inhibiting the growth of other pathogenic bacteria in the gastrointestinal tract such as <i>Salmonella typhimurium</i> and <i>Escherichia coli.</i> Most of the current synthetic expression systems for LL-37 depend on the construction of soluble fusion partners to avoid cytotoxic effects of the antimicrobial peptide in the <i>E. coli</i> host strain. However, these fusion systems require additional cleavage steps using enzymatic or chemical methods, which makes them impossible to express an active LL-37 peptide in vivo. In order to create a resistant host that can export LL-37 to the media, we intend to overexpress the <i>E. coli</i> acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to this and similar antimicrobial peptides by expulsion. Our aim is to create a system in which <i>E. coli</i> expels LL-37 only when H. pylori or other pathogenic bacteria, such as <i>S. typhimurium, E. coli</i> or <i>V. cholerae</i>, are present. In order to do this we are using the LsrA promoter, which allows transcription in the presence of AI-2, a molecule produced by bacteria to communicate via quorum-sensing. <i>E. coli</i> also produces AI-2, so we designed an antisense RNA with specific secondary structure to inhibit the translation of LuxS, the enzyme responsible of the production of AI-2 in <i>E. coli</i>. </p>
-
<div id="u0" class="u0_container" >
+
-
 
+
-
<div id="u0_img" class="u0_normal detectCanvas"></div>
+
-
 
+
-
<div id="u1" class="u1" style="visibility:hidden;" >
+
-
 
+
-
<div id="u1_rtf"></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<DIV id="u2container" style="position:absolute; left:160px; top:130px; width:940px; height:60px;; overflow:visible">
+
-
 
+
-
<DIV id="u2_menu" class="u2_menu detectCanvas"></DIV>
+
-
 
+
-
 
+
-
 
+
-
<DIV id="u3" class="u3;" >
+
-
 
+
-
 
+
-
 
+
-
</DIV>
+
-
 
+
-
 
+
-
 
+
-
<DIV id="u3container" style="position:absolute; left:2px; top:2px; width:941px; height:61px; overflow:visible">
+
-
 
+
-
 
+
-
 
+
-
<div id="u4" class="u4_container" >
+
-
 
+
-
<div id="u4_img" class="u4_normal detectCanvas"></div>
+
-
 
+
-
<div id="u5" class="u5" >
+
-
 
+
-
<div id="u5_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">HOME</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u6" class="u6_container" >
+
-
 
+
-
<div id="u6_img" class="u6_normal detectCanvas"></div>
+
-
 
+
-
<div id="u7" class="u7" >
+
-
 
+
-
<div id="u7_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">PROJECT</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u8" class="u8_container" >
+
-
 
+
-
<div id="u8_img" class="u8_normal detectCanvas"></div>
+
-
 
+
-
<div id="u9" class="u9" >
+
-
 
+
-
<div id="u9_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">HUMAN PRACTICES </span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u10" class="u10_container" >
+
-
 
+
-
<div id="u10_img" class="u10_normal detectCanvas"></div>
+
-
 
+
-
<div id="u11" class="u11" >
+
-
 
+
-
<div id="u11_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">TEAM</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u12" class="u12_container" >
+
-
 
+
-
<div id="u12_img" class="u12_normal detectCanvas"></div>
+
-
 
+
-
<div id="u13" class="u13" >
+
-
 
+
-
<div id="u13_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">SAFETY</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</DIV>
+
-
 
+
-
</DIV>
+
-
 
+
-
<DIV id="sm0u2" style="position:absolute; left:160px; top:130px; width:940px; height:60px; visibility:hidden; width:1px; height:1px;" >
+
-
 
+
-
<DIV id="u14container" style="position:absolute; left:152px; top:58px; width:150px; height:184px;; overflow:visible">
+
-
 
+
-
 
+
-
 
+
-
<DIV id="u14_menu" class="u14_menu detectCanvas"></DIV>
+
-
 
+
-
 
+
-
 
+
-
<DIV id="u15" class="u15;" >
+
-
 
+
-
 
+
-
 
+
-
</DIV>
+
-
 
+
-
 
+
-
 
+
-
<DIV id="u15container" style="position:absolute; left:2px; top:2px; width:151px; height:185px; overflow:visible">
+
-
 
+
-
 
+
-
 
+
-
<div id="u16" class="u16_container" >
+
-
 
+
-
<div id="u16_img" class="u16_normal detectCanvas"></div>
+
-
 
+
-
<div id="u17" class="u17" >
+
-
 
+
-
<div id="u17_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Overview</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u18" class="u18_container" >
+
-
 
+
-
<div id="u18_img" class="u18_normal detectCanvas"></div>
+
-
 
+
-
<div id="u19" class="u19" >
+
-
 
+
-
<div id="u19_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">General Design</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u20" class="u20_container" >
+
-
 
+
-
<div id="u20_img" class="u20_normal detectCanvas"></div>
+
-
 
+
-
<div id="u21" class="u21" >
+
-
 
+
-
<div id="u21_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Wet Lab</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u22" class="u22_container" >
+
-
 
+
-
<div id="u22_img" class="u22_normal detectCanvas"></div>
+
-
 
+
-
<div id="u23" class="u23" >
+
-
 
+
-
<div id="u23_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Dry Lab</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u24" class="u24_container" >
+
-
 
+
-
<div id="u24_img" class="u24_normal detectCanvas"></div>
+
-
 
+
-
<div id="u25" class="u25" >
+
-
 
+
-
<div id="u25_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Conclusions</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u26" class="u26_container" >
+
-
 
+
-
<div id="u26_img" class="u26_normal detectCanvas"></div>
+
-
 
+
-
<div id="u27" class="u27" >
+
-
 
+
-
<div id="u27_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">References</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</DIV>
+
-
 
+
-
</DIV>
+
-
 
+
-
</DIV>
+
-
 
+
-
<script type="text/javascript">
+
-
 
+
-
InitializeSubmenu('sm0u2', 'u6');
+
-
 
+
-
</script>
+
-
 
+
-
<DIV id="sm1u2" style="position:absolute; left:160px; top:130px; width:940px; height:60px; visibility:hidden; width:1px; height:1px;" >
+
-
 
+
-
<DIV id="u28container" style="position:absolute; left:552px; top:58px; width:151px; height:64px;; overflow:visible">
+
-
 
+
-
 
+
-
 
+
-
<DIV id="u28_menu" class="u28_menu detectCanvas"></DIV>
+
-
 
+
-
 
+
-
 
+
-
<DIV id="u29" class="u29;" >
+
-
 
+
-
 
+
-
 
+
-
</DIV>
+
-
 
+
-
 
+
-
 
+
-
<DIV id="u29container" style="position:absolute; left:2px; top:2px; width:152px; height:65px; overflow:visible">
+
-
 
+
-
 
+
-
 
+
-
<div id="u30" class="u30_container" >
+
-
 
+
-
<div id="u30_img" class="u30_normal detectCanvas"></div>
+
-
 
+
-
<div id="u31" class="u31" >
+
-
 
+
-
<div id="u31_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Bios</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u32" class="u32_container" >
+
-
 
+
-
<div id="u32_img" class="u32_normal detectCanvas"></div>
+
-
 
+
-
<div id="u33" class="u33" >
+
-
 
+
-
<div id="u33_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Sponsors</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</DIV>
+
-
 
+
-
</DIV>
+
-
 
+
-
</DIV>
+
-
 
+
-
<script type="text/javascript">
+
-
 
+
-
InitializeSubmenu('sm1u2', 'u10');
+
-
 
+
-
</script>
+
-
 
+
-
<DIV id="sm2u2" style="position:absolute; left:160px; top:130px; width:940px; height:60px; visibility:hidden; width:1px; height:1px;" >
+
-
 
+
-
<DIV id="u34container" style="position:absolute; left:302px; top:58px; width:178px; height:94px;; overflow:visible">
+
-
 
+
-
 
+
-
 
+
-
<DIV id="u34_menu" class="u34_menu detectCanvas"></DIV>
+
-
 
+
-
 
+
-
 
+
-
<DIV id="u35" class="u35;" >
+
-
 
+
-
 
+
-
 
+
-
</DIV>
+
-
 
+
-
 
+
-
 
+
-
<DIV id="u35container" style="position:absolute; left:2px; top:2px; width:179px; height:95px; overflow:visible">
+
-
 
+
-
 
+
-
 
+
-
<div id="u36" class="u36_container" >
+
-
 
+
-
<div id="u36_img" class="u36_normal detectCanvas"></div>
+
-
 
+
-
<div id="u37" class="u37" >
+
-
 
+
-
<div id="u37_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Overview</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u38" class="u38_container" >
+
-
 
+
-
<div id="u38_img" class="u38_normal detectCanvas"></div>
+
-
 
+
-
<div id="u39" class="u39" >
+
-
 
+
-
<div id="u39_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Journalism</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u40" class="u40_container" >
+
-
 
+
-
<div id="u40_img" class="u40_normal detectCanvas"></div>
+
-
 
+
-
<div id="u41" class="u41" >
+
-
 
+
-
<div id="u41_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Interviews</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</DIV>
+
-
 
+
-
</DIV>
+
-
 
+
-
</DIV>
+
-
 
+
-
<script type="text/javascript">
+
-
 
+
-
InitializeSubmenu('sm2u2', 'u8');
+
-
 
+
-
</script>
+
-
 
+
-
<div id="u42" class="u42_container" >
+
-
 
+
-
<div id="u42_img" class="u42_normal detectCanvas"></div>
+
-
 
+
-
<div id="u43" class="u43" style="visibility:hidden;" >
+
-
 
+
-
<div id="u43_rtf"></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u44" ></div>
+
-
 
+
-
<DIV id="u45" style="border-style:dotted; border-color:red; visibility:hidden; position:absolute; left:160px; top:20px; width:940px; height:1620px;;" ></div><div id="u46" class="u46" >
+
-
 
+
-
<DIV id="u46_line" class="u46_line" ></DIV>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u47" class="u47" >
+
-
 
+
-
<div id="u47_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:20px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">THE PROJECT</span></p></div>
+
-
 
+
-
</div><div id="u48" class="u48" >
+
-
 
+
-
<DIV id="u48_line" class="u48_line" ></DIV>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u49" class="u49" >
+
-
 
+
-
<div id="u49_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:20px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">THE PEOPLE</span></p></div>
+
-
 
+
-
</div><div id="u50" class="u50" >
+
-
 
+
-
<DIV id="u50_line" class="u50_line" ></DIV>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u51" class="u51" >
+
-
 
+
-
<div id="u51_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:20px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">PARTS</span></p></div>
+
-
 
+
-
</div><div id="u52" class="u52" >
+
-
 
+
-
<DIV id="u52_line" class="u52_line" ></DIV>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u53" class="u53" >
+
-
 
+
-
<div id="u53_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:20px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">ACHIEVEMENTS</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u54" class="u54" >
+
-
 
+
-
<div id="u54_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Praesent libero arcu, convallis et dapibus sit amet, aliquet nec nibh. Nulla eu aliquam orci. Phasellus vitae nunc ut urna tincidunt accumsan vel ac dui. Donec tempus quis quam vitae ultrices. Vestibulum congue nec odio eget dictum. Sed tincidunt velit enim. Fusce metus augue, lobortis eget viverra sit amet, fringilla.</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u55" class="u55" >
+
-
 
+
-
<div id="u55_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Praesent libero arcu, convallis et dapibus sit amet, aliquet nec nibh. Nulla eu aliquam orci. Phasellus vitae nunc ut urna tincidunt accumsan vel ac dui. Donec tempus quis quam vitae ultrices. Vestibulum congue nec odio eget dictum. Sed tincidunt velit enim. Fusce metus augue, lobortis eget viverra sit amet, fringilla.</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u56" class="u56" >
+
-
 
+
-
<div id="u56_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Praesent libero arcu, convallis et dapibus sit amet, aliquet nec nibh. Nulla eu aliquam orci. Phasellus vitae nunc ut urna tincidunt accumsan vel ac dui. Donec tempus quis quam vitae ultrices. Vestibulum congue nec odio eget dictum. Sed tincidunt velit enim. Fusce metus augue, lobortis eget viverra sit amet, fringilla.</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u57" class="u57" >
+
-
 
+
-
<div id="u57_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Praesent libero arcu, convallis et dapibus sit amet, aliquet nec nibh. Nulla eu aliquam orci. Phasellus vitae nunc ut urna tincidunt accumsan vel ac dui. Donec tempus quis quam vitae ultrices. Vestibulum congue nec odio eget dictum. Sed tincidunt velit enim. Fusce metus augue, lobortis eget viverra sit amet, fringilla.</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u58" class="u58" >
+
-
 
+
-
<div id="u58_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Read the abstract</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u59" class="u59" >
+
-
 
+
-
<div id="u59_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Meet the team</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u60" class="u60" >
+
-
 
+
-
<div id="u60_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Read more...</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u61" class="u61" >
+
-
 
+
-
<div id="u61_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Read more...</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u62" class="u62_container" >
+
-
 
+
-
<div id="u62_img" >
+
-
 
+
-
<img src="https://static.igem.org/mediawiki/2013/3/3b/U62_normal.jpg" class="raw_image"></div>
+
-
 
+
-
<div id="u63" class="u63" style="visibility:hidden;" >
+
-
 
+
-
<div id="u63_rtf"></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u64" class="u64_container" >
+
-
 
+
-
<div id="u64_img" >
+
-
 
+
-
<img src="https://static.igem.org/mediawiki/2013/a/ae/U64_normal.jpg" class="raw_image"></div>
+
-
 
+
-
<div id="u65" class="u65" style="visibility:hidden;" >
+
-
 
+
-
<div id="u65_rtf"></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u66" class="u66_container" >
+
-
 
+
-
<div id="u66_img" >
+
-
 
+
-
<img src="https://static.igem.org/mediawiki/2013/d/d3/U66_normal.jpg" class="raw_image"></div>
+
-
 
+
-
<div id="u67" class="u67" style="visibility:hidden;" >
+
-
 
+
-
<div id="u67_rtf"></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u68" class="u68_container" >
+
-
 
+
-
<div id="u68_img" >
+
-
 
+
-
<img src="https://static.igem.org/mediawiki/2013/3/30/U68_normal.jpg" class="raw_image"></div>
+
-
 
+
-
<div id="u69" class="u69" style="visibility:hidden;" >
+
-
 
+
-
<div id="u69_rtf"></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u70" class="u70_container" >
+
-
 
+
-
<div id="u70_img" >
+
-
 
+
-
<img src="https://static.igem.org/mediawiki/2013/5/55/U70_normal.gif" class="raw_image"></div>
+
-
 
+
-
<div id="u71" class="u71" style="visibility:hidden;" >
+
-
 
+
-
<div id="u71_rtf"></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u72" class="u72_container" >
+
-
 
+
-
<div id="u72_img" >
+
-
 
+
-
<img src="https://static.igem.org/mediawiki/2013/d/d1/U72_normal.jpg" class="raw_image"></div>
+
-
 
+
-
<div id="u73" class="u73" style="visibility:hidden;" >
+
-
 
+
-
<div id="u73_rtf"></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div><div id="u74" class="u74" >
+
-
 
+
-
<DIV id="u74_line" class="u74_line" ></DIV>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u75" class="u75" >
+
-
 
+
-
<div id="u75_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:28px;font-weight:bold;font-style:normal;text-decoration:none;color:#4B4F54;">News from the lab</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="u76" class="u76" >
+
-
 
+
-
<div id="u76_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:28px;font-weight:bold;font-style:normal;text-decoration:none;color:#4B4F54;">Gallery</span></p></div>
+
-
 
+
-
</div>
+
-
 
+
-
<DIV id="u77" style="" data-label="Rotating Photos">
+
-
 
+
-
<DIV id="pd2u77" style="visibility:hidden;" data-label="State3">
+
-
 
+
-
<div id="u78" class="u78_container" >
+
-
 
+
-
<div id="u78_img" class="u78_normal detectCanvas"></div>
+
-
 
+
-
<div id="u79" class="u79" style="visibility:hidden;" >
+
-
 
+
-
<div id="u79_rtf"></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</DIV>
+
-
 
+
-
<DIV id="pd1u77" style="visibility:hidden;" data-label="State2">
+
-
 
+
-
<div id="u80" class="u80_container" >
+
-
 
+
-
<div id="u80_img" class="u80_normal detectCanvas"></div>
+
-
 
+
-
<div id="u81" class="u81" style="visibility:hidden;" >
+
-
 
+
-
<div id="u81_rtf"></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</DIV>
+
-
 
+
-
<DIV id="pd0u77" style="" data-label="State1">
+
-
 
+
-
<div id="u82" class="u82_container" >
+
-
 
+
-
<div id="u82_img" class="u82_normal detectCanvas"></div>
+
-
 
+
-
<div id="u83" class="u83" style="visibility:hidden;" >
+
-
 
+
-
<div id="u83_rtf"></div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</DIV>
+
-
 
+
-
</DIV><div id="u84" class="u84" >
+
-
 
+
-
<DIV id="u84_line" class="u84_line" ></DIV>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div class="preload"><img src="https://static.igem.org/mediawiki/2013/d/d1/Bg_ciencias.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/5/59/U0_normal.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/c/c3/U2_menu.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/8/88/U4_mouseOver.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/9/96/U4_normal.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/8/84/U8_mouseOver.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/6/6f/U8_normal.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/c/ca/U12_mouseOver.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/e/e3/U12_normal.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/0/0a/U14_menu.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/2/2d/U16_normal.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/5/55/U28_menu.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/6/68/U30_mouseOver.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/f/f7/U30_normal.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/6/6c/U34_menu.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/7/72/U36_mouseOver.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/3/30/U36_normal.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/0/0a/U42_normal.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/b/b8/U46_line.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/f/fb/U52_line.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/f/fb/U52_line.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/f/fb/U52_line.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/3/3b/U62_normal.jpg" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/a/ae/U64_normal.jpg" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/d/d3/U66_normal.jpg" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/3/30/U68_normal.jpg" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/5/55/U70_normal.gif" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/d/d1/U72_normal.jpg" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/8/80/U74_line.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/4/45/U78_normal.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/6/6a/U80_normal.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/9/94/U82_normal.png" width="1" height="1"/><img src="https://static.igem.org/mediawiki/2013/a/a6/U84_line.png" width="1" height="1"/></div>
+
-
 
+
</body>
</body>
-
 
+
</html>
-
<script src="https://2013.igem.org/Team:Ciencias-UNAM/scripts/pagescript?action=raw&ctype=text/javascript"></script>
+
-
 
+
-
<script src="https://2013.igem.org/Team:Ciencias-UNAM/scripts/pagescript_Home?action=raw&ctype=text/javascript" charset="utf-8"></script>
+

Revision as of 02:50, 12 August 2013

Abstract

The human peptide LL-37 is a cationic antimicrobial peptide with activity against Gram-negative and Gram-positive bacteria. It has been shown to protect against Helicobacter pylori infection and its related gastritis, besides of inhibiting the growth of other pathogenic bacteria in the gastrointestinal tract such as Salmonella typhimurium and Escherichia coli. Most of the current synthetic expression systems for LL-37 depend on the construction of soluble fusion partners to avoid cytotoxic effects of the antimicrobial peptide in the E. coli host strain. However, these fusion systems require additional cleavage steps using enzymatic or chemical methods, which makes them impossible to express an active LL-37 peptide in vivo. In order to create a resistant host that can export LL-37 to the media, we intend to overexpress the E. coli acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to this and similar antimicrobial peptides by expulsion. Our aim is to create a system in which E. coli expels LL-37 only when H. pylori or other pathogenic bacteria, such as S. typhimurium, E. coli or V. cholerae, are present. In order to do this we are using the LsrA promoter, which allows transcription in the presence of AI-2, a molecule produced by bacteria to communicate via quorum-sensing. E. coli also produces AI-2, so we designed an antisense RNA with specific secondary structure to inhibit the translation of LuxS, the enzyme responsible of the production of AI-2 in E. coli.

Retrieved from "http://2013.igem.org/Team:Ciencias-UNAM"