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Team:DTU-Denmark/Notebook/12 August 2013
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==Main purpose== | ==Main purpose== | ||
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| - | # PCR reaction to amplify backbone pZA21 containing araBAD and RFP | + | # PCR reaction in order to amplify backbone pZA21 containing araBAD and RFP |
# PCR reaction in order to amplify Nir1 and Nir2 fragments | # PCR reaction in order to amplify Nir1 and Nir2 fragments | ||
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==Procedure== | ==Procedure== | ||
<hr/> | <hr/> | ||
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| + | # PCR mix for backbone was made according to standard protocol. | ||
| + | *Primers - 1an and 1b | ||
| + | * Template - "Ara" sample (plasmid isolation pZA21:araBAD:RFP). | ||
| + | * Program was based on standard program with 65 C of annealing temperature and 4 min of extension time. | ||
| + | * Samples names: 1, 2, 3 and N (negative) | ||
| + | |||
| + | # PCR for Nir1 and Nir2 | ||
==Results== | ==Results== | ||
Revision as of 15:25, 12 August 2013
12 August 2013
Contents |
lab 208
Main purpose
- PCR reaction in order to amplify backbone pZA21 containing araBAD and RFP
- PCR reaction in order to amplify Nir1 and Nir2 fragments
Who was in the lab
Henrike, Kristian, Gosia
Procedure
- PCR mix for backbone was made according to standard protocol.
- Primers - 1an and 1b
- Template - "Ara" sample (plasmid isolation pZA21:araBAD:RFP).
- Program was based on standard program with 65 C of annealing temperature and 4 min of extension time.
- Samples names: 1, 2, 3 and N (negative)
- PCR for Nir1 and Nir2
Results
Conclusion
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