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Team:DTU-Denmark/Notebook/12 August 2013
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| - | # PCR mix for backbone | + | ===# PCR mix for backbone=== |
| - | *Primers - 1an and 1b | + | * PCR mix - according to standard protocol. |
| + | * Primers - 1an and 1b | ||
* Template - "Ara" sample (plasmid isolation pZA21:araBAD:RFP). | * Template - "Ara" sample (plasmid isolation pZA21:araBAD:RFP). | ||
* Program was based on standard program with 65 C of annealing temperature and 4 min of extension time. | * Program was based on standard program with 65 C of annealing temperature and 4 min of extension time. | ||
* Samples names: 1, 2, 3 and N (negative) | * Samples names: 1, 2, 3 and N (negative) | ||
| - | # PCR for Nir1 and Nir2 | + | ===# PCR for Nir1 and Nir2=== |
==Results== | ==Results== | ||
Revision as of 15:26, 12 August 2013
12 August 2013
Contents |
lab 208
Main purpose
- PCR reaction in order to amplify backbone pZA21 containing araBAD and RFP
- PCR reaction in order to amplify Nir1 and Nir2 fragments
Who was in the lab
Henrike, Kristian, Gosia
Procedure
# PCR mix for backbone
- PCR mix - according to standard protocol.
- Primers - 1an and 1b
- Template - "Ara" sample (plasmid isolation pZA21:araBAD:RFP).
- Program was based on standard program with 65 C of annealing temperature and 4 min of extension time.
- Samples names: 1, 2, 3 and N (negative)
# PCR for Nir1 and Nir2
Results
Conclusion
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