Revision as of 15:35, 12 August 2013

Notebook : August 12

Obtaining Pfnr_RBS-LacZ-Term in PSB1C3

Anaïs, Nadia, XiaoJing

Protocol : digestion

Picture: lysed cells comparison.

0µl phage(control): the petri dish is cloudy, bacteria are not lysed.
50µl phage: the petri dish is clear, bacteria are lysed by phages.
We used a wild type strain to keep a stock and a Δ fnr E. coli strain to obtain phages that might have encapsidated a Δfnr::Km fragment.

10µl,50µl and 100µl petri dishes are clear so phages are multiplied.

We let the antibiotic over night to select the right strain.

Abdou

Clone 10,14 and 15 plamid extraction using nucleospin kit.

Results: concentration measured by nanodrop

We still have to sequence plasmids in order to verify our results.

August 1st PCR purification to be sure about the experiment. BphR2 part1, BphR2 part2 and RBS_BphR2_part1, FNR part1, FNR part2 and RBS_FNR_part1.

Results:

IMAGE

we have no fragment so we must do again these PCRs

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+='''Notebook : August 12'''=
 =='''Lab work'''==
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+Obtaining Pfnr_RBS-LacZ-Term in PSB1C3
+===='''1 - Digestion of Bba_K1155000, Bba_K1155007 and Bba_K1155003'''====
 Anaïs, Nadia, XiaoJing
 Protocol : [[Team:Paris_Saclay/Protocols/digestion|digestion]]