Team:Groningen/Labwork/12 August 2013

From 2013.igem.org

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<br>The normal test was done in duplo (due to a failed abstacle preparation). the other test are 3 ml agar on top of the normal level.
<br>The normal test was done in duplo (due to a failed abstacle preparation). the other test are 3 ml agar on top of the normal level.
And a cone (salvaged from a 5 ml pipet) seperating the strain from air.
And a cone (salvaged from a 5 ml pipet) seperating the strain from air.
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<br>Tubes are to grow overnight in a 37C incubator.
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<br>Tubes are to grow overnight in a 37C incubator. Except one of the duplo series which was put in the shaker to see the difference.
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Revision as of 19:40, 12 August 2013

Sander

did a miniprep of CFP 1,2 and 4 and Pdes CheY 1 and 2 and a gell purification of Munich EXP

Inne

Made -80 stock of strains OI3017 OI3059 OI2653 OI3135.
Made a setup to test motility, using 0.4% agar test-tubes with different obstacles in them.
4 sets of 3 tubes are stabbed with a toothpick, one negative, one with Bacillus subtillus gfp backbone, and one OI3017.
The OI3017 should not be motile the Bacillus subtillus gfp should be motile and the negative control should be negative.
The normal test was done in duplo (due to a failed abstacle preparation). the other test are 3 ml agar on top of the normal level. And a cone (salvaged from a 5 ml pipet) seperating the strain from air.
Tubes are to grow overnight in a 37C incubator. Except one of the duplo series which was put in the shaker to see the difference.