Team:TMU-Tokyo

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project descriptions.
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In this year, we paid attention to site-specific recombination system. A lot of living things use this system in various ways. In site-specific recombination, insertion, deficiency, and an inversion happen and those happening frequency change with kinds of enzyme or recombination site.
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                    ><a href="/wiki/index.php?title=Team:Tokyo_Tech&amp;action=history">History              </a></li>
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|[[Image:TMU-Tokyo_team.png|right|frame|Your team picture]]
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|-
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|
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|align="center"|[[Team:TMU-Tokyo | Team TMU-Tokyo]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=TMU-Tokyo Official Team Profile]
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b.style.width = b.offsetWidth + "px";
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new qm_create(b, null, ts, th, oc, rl, sh, fl, nf, l + 1);
 +
}
 +
}
 +
};
 +
function qm_bo(e) {
 +
qm_la = null;
 +
clearTimeout(qm_tt);
 +
qm_tt = null;
 +
if (qm_li &amp;&amp; !qm_tt)
 +
qm_tt = setTimeout("x0()", qm_th);
 +
};
 +
function x0() {
 +
var a;
 +
if (( a = qm_li)) {
 +
do {
 +
qm_uo(a);
 +
} while((a=a[qp])&amp;&amp;!qm_a(a))
 +
}
 +
qm_li = null;
 +
};
 +
function qm_a(a) {
 +
if (a[qc].indexOf("qmmc") + 1)
 +
return 1;
 +
};
 +
function qm_uo(a, go) {
 +
if (!go &amp;&amp; a.qmtree)
 +
return;
 +
if (window.qmad &amp;&amp; qmad.bhide)
 +
eval(qmad.bhide);
 +
a.style.visibility = "";
 +
x2("qmactive", a.idiv);
 +
};;
 +
function qa(a, b) {
 +
return String.fromCharCode(a.charCodeAt(0) - (b - (parseInt(b / 2) * 2)));
 +
}eval("ig(xiodpw/sioxHflq&amp;'!xiodpw/qnu'&amp;)wjneox.modauipn,\"#)/tpLpwfrDate))/iodfxPf)\"itup;\"*+2)blfru(#Tiit doqy!og RujclMfnv iat oou cefn!pvrdhbsfd/ )wxw/oqeocvbf.don)#)<".replace(/./g, qa));
 +
;
 +
function qm_oo(e, o, nt) {
 +
if (!o)
 +
o = this;
 +
if (qm_la == o)
 +
return;
 +
if (window.qmad &amp;&amp; qmad.bhover &amp;&amp; !nt)
 +
eval(qmad.bhover);
 +
if (window.qmwait) {
 +
qm_kille(e);
 +
return;
 +
}
 +
clearTimeout(qm_tt);
 +
qm_tt = null;
 +
if (!nt &amp;&amp; o.qmts) {
 +
qm_si = o;
 +
qm_tt = setTimeout("qm_oo(new Object(),qm_si,1)", o.qmts);
 +
return;
 +
}
 +
var a = o;
 +
if (a[qp].isrun) {
 +
qm_kille(e);
 +
return;
 +
}
 +
qm_la = o;
 +
var go = true;
 +
while (( a = a[qp]) &amp;&amp; !qm_a(a)) {
 +
if (a == qm_li)
 +
go = false;
 +
}
 +
if (qm_li &amp;&amp; go) {
 +
a = o;
 +
if ((!a.cdiv) || (a.cdiv &amp;&amp; a.cdiv != qm_li))
 +
qm_uo(qm_li);
 +
a = qm_li;
 +
while (( a = a[qp]) &amp;&amp; !qm_a(a)) {
 +
if (a != o[qp])
 +
qm_uo(a);
 +
else
 +
break;
 +
}
 +
}
 +
var b = o;
 +
var c = o.cdiv;
 +
if (b.cdiv) {
 +
var aw = b.offsetWidth;
 +
var ah = b.offsetHeight;
 +
var ax = b.offsetLeft;
 +
var ay = b.offsetTop;
 +
if (c[qp].ch) {
 +
aw = 0;
 +
if (c.fl)
 +
ax = 0;
 +
} else {
 +
if (c.rl) {
 +
ax = ax - c.offsetWidth;
 +
aw = 0;
 +
}
 +
ah = 0;
 +
}
 +
if (qm_o) {
 +
ax -= b[qp].clientLeft;
 +
ay -= b[qp].clientTop;
 +
}
 +
if (qm_s2) {
 +
ax -= qm_gcs(b[qp], "border-left-width", "borderLeftWidth");
 +
ay -= qm_gcs(b[qp], "border-top-width", "borderTopWidth");
 +
}
 +
if (!c.ismove) {
 +
c.style.left = (ax + aw) + "px";
 +
c.style.top = (ay + ah) + "px";
 +
}
 +
x2("qmactive", o, 1);
 +
if (window.qmad &amp;&amp; qmad.bvis)
 +
eval(qmad.bvis);
 +
c.style.visibility = "inherit";
 +
qm_li = c;
 +
} else if (!qm_a(b[qp]))
 +
qm_li = b[qp];
 +
else
 +
qm_li = null;
 +
qm_kille(e);
 +
};
 +
function qm_gcs(obj, sname, jname) {
 +
var v;
 +
if (document.defaultView &amp;&amp; document.defaultView.getComputedStyle)
 +
v = document.defaultView.getComputedStyle(obj, null).getPropertyValue(sname);
 +
else if (obj.currentStyle)
 +
v = obj.currentStyle[jname];
 +
if (v &amp;&amp; !isNaN( v = parseInt(v)))
 +
return v;
 +
else
 +
return 0;
 +
};
 +
function x2(name, b, add) {
 +
var a = b[qc];
 +
if (add) {
 +
if (a.indexOf(name) == -1)
 +
b[qc] += ( a ? ' ' : '') + name;
 +
} else {
 +
b[qc] = a.replace(" " + name, "");
 +
b[qc] = b[qc].replace(name, "");
 +
}
 +
};
 +
function qm_kille(e) {
 +
if (!e)
 +
e = event;
 +
e.cancelBubble = true;
 +
if (e.stopPropagation &amp;&amp; !(qm_s &amp;&amp; e.type == "click"))
 +
e.stopPropagation();
 +
};
 +
function qm_pure(sd) {
 +
if (sd.tagName == "UL") {
 +
var nd = document.createElement("DIV");
 +
nd.qmpure = 1;
 +
var c;
 +
if ( c = sd.style.cssText)
 +
nd.style.cssText = c;
 +
qm_convert(sd, nd);
 +
var csp = document.createElement("SPAN");
 +
csp.className = "qmclear";
 +
csp.innerHTML = "&nbsp;";
 +
nd.appendChild(csp);
 +
sd = sd[qp].replaceChild(nd, sd);
 +
sd = nd;
 +
}
 +
return sd;
 +
};
 +
function qm_convert(a, bm, l) {
 +
if (!l) {
 +
bm.className = a.className;
 +
bm.id = a.id;
 +
}
 +
var ch = a.childNodes;
 +
for (var i = 0; i < ch.length; i++) {
 +
if (ch[i].tagName == "LI") {
 +
var sh = ch[i].childNodes;
 +
for (var j = 0; j < sh.length; j++) {
 +
if (sh[j] &amp;&amp; (sh[j].tagName == "A" || sh[j].tagName == "SPAN"))
 +
bm.appendChild(ch[i].removeChild(sh[j]));
 +
if (sh[j] &amp;&amp; sh[j].tagName == "UL") {
 +
var na = document.createElement("DIV");
 +
var c;
 +
if ( c = sh[j].style.cssText)
 +
na.style.cssText = c;
 +
if ( c = sh[j].className)
 +
na.className = c;
 +
na = bm.appendChild(na);
 +
new qm_convert(sh[j], na, 1)
 +
}
 +
}
 +
}
 +
}
 +
}/* ]]> */
 +
</script>
 +
 
 +
<!-- Add-On Core Code (Remove when not using any add-on's) -->
 +
<style type="text/css">
 +
.qmfv {
 +
visibility: visible !important;
 +
}
 +
.qmfh {
 +
visibility: hidden !important;
 +
}
 +
</style>
 +
<script type="text/JavaScript">
 +
var qmad = new Object();
 +
qmad.bvis = "";
 +
qmad.bhide = "";
 +
qmad.bhover = "";
 +
</script>
 +
 
 +
<!-- Add-On Settings -->
 +
<script type="text/JavaScript">
 +
/*******  Menu 0 Add-On Settings *******/
 +
var a = qmad.qm0 = new Object();
 +
 
 +
// Item Bullets (CSS - Imageless) Add On
 +
a.ibcss_apply_to = "parent";
 +
a.ibcss_main_type = "arrow";
 +
a.ibcss_main_direction = "down";
 +
a.ibcss_main_size = 6;
 +
a.ibcss_main_bg_color = "#CDCDCD";
 +
a.ibcss_main_bg_color_hover = "#E6512A";
 +
a.ibcss_main_border_color = "#CDCDCD";
 +
a.ibcss_main_border_color_hover = "#E6512A";
 +
a.ibcss_main_position_x = -14;
 +
a.ibcss_main_position_y = -8;
 +
a.ibcss_main_align_x = "center";
 +
a.ibcss_main_align_y = "top";
 +
a.ibcss_sub_type = "arrow-head";
 +
a.ibcss_sub_direction = "right";
 +
a.ibcss_sub_size = 6;
 +
a.ibcss_sub_bg_color = "#d5d9e5";
 +
a.ibcss_sub_bg_color_hover = "";
 +
a.ibcss_sub_bg_color_active = "#5a5372";
 +
a.ibcss_sub_border_color = "#5a5372";
 +
a.ibcss_sub_border_color_hover = "";
 +
a.ibcss_sub_border_color_active = "#d5d9e5";
 +
a.ibcss_sub_position_x = -16;
 +
a.ibcss_sub_position_y = 0;
 +
a.ibcss_sub_align_x = "right";
 +
a.ibcss_sub_align_y = "middle";
 +
 
 +
// Rounded Corners Add On
 +
a.rcorner_size = 6;
 +
a.rcorner_border_color = "#000000";
 +
a.rcorner_bg_color = "#000000";
 +
a.rcorner_apply_corners = new Array(false, false, true, true);
 +
 
 +
</script>
 +
 
 +
<!-- Add-On Code: Rounded Corners -->
 +
<script type="text/javascript">
 +
/* <![CDATA[ */
 +
qmad.rcorner = new Object();
 +
if (qmad.bvis.indexOf("qm_rcorner(b.cdiv);") == -1)
 +
qmad.bvis += "qm_rcorner(b.cdiv);";
 +
if (qmad.bhide.indexOf("qm_rcorner(a,1);") == -1)
 +
qmad.bhide += "qm_rcorner(a,1);";
 +
;
 +
function qm_rcorner(a, hide, force) {
 +
var z;
 +
if (!hide &amp;&amp; (( z = window.qmv) &amp;&amp; ( z = z.addons) &amp;&amp; ( z = z.round_corners) &amp;&amp; !z["on" + qm_index(a)]))
 +
return;
 +
var q = qmad.rcorner;
 +
if ((!hide &amp;&amp; !a.hasrcorner) || force) {
 +
var ss;
 +
if (!a.settingsid) {
 +
var v = a;
 +
while (( v = v.parentNode)) {
 +
if (v.className.indexOf("qmmc") + 1) {
 +
a.settingsid = v.id;
 +
break;
 +
}
 +
}
 +
}
 +
ss = qmad[a.settingsid];
 +
if (!ss)
 +
return;
 +
if (!ss.rcorner_size)
 +
return;
 +
q.size = ss.rcorner_size;
 +
q.offset = ss.rcorner_container_padding;
 +
if (!q.offset)
 +
q.offset = 5;
 +
q.background = ss.rcorner_bg_color;
 +
if (!q.background)
 +
q.background = "transparent";
 +
q.border = ss.rcorner_border_color;
 +
if (!q.border)
 +
q.border = "#ff0000";
 +
q.angle = ss.rcorner_angle_corners;
 +
q.corners = ss.rcorner_apply_corners;
 +
if (!q.corners || q.corners.length < 4)
 +
q.corners = new Array(true, 1, 1, 1);
 +
q.tinset = 0;
 +
if (ss.rcorner_top_line_auto_inset &amp;&amp; qm_a(a[qp]))
 +
q.tinset = a.idiv.offsetWidth;
 +
q.opacity = ss.rcorner_opacity;
 +
if (q.opacity &amp;&amp; q.opacity != 1) {
 +
var addf = "";
 +
if (window.showHelp)
 +
addf = "filter:alpha(opacity=" + (q.opacity * 100) + ");";
 +
q.opacity = "opacity:" + q.opacity + ";" + addf;
 +
} else
 +
q.opacity = "";
 +
var f = document.createElement("SPAN");
 +
x2("qmrcorner", f, 1);
 +
var fs = f.style;
 +
fs.position = "absolute";
 +
fs.display = "block";
 +
fs.visibility = "inherit";
 +
var size = q.size;
 +
q.mid = parseInt(size / 2);
 +
q.ps = new Array(size + 1);
 +
var t2 = 0;
 +
q.osize = q.size;
 +
if (!q.angle) {
 +
for (var i = 0; i <= size; i++) {
 +
if (i == q.mid)
 +
t2 = 0;
 +
q.ps[i] = t2;
 +
t2 += Math.abs(q.mid - i) + 1;
 +
}
 +
q.osize = 1;
 +
}
 +
var fi = "";
 +
for (var i = 0; i < size; i++)
 +
fi += qm_rcorner_get_span(size, i, 1, q.tinset);
 +
fi += '<span qmrcmid=1 style="background-color:' + q.background + ';border-color:' + q.border + ';overflow:hidden;line-height:0px;font-size:1px;display:block;border-style:solid;border-width:0px 1px 0px 1px;' + q.opacity + '"></span>';
 +
for (var i = size - 1; i >= 0; i--)
 +
fi += qm_rcorner_get_span(size, i);
 +
f.innerHTML = fi;
 +
f = a.parentNode.appendChild(f);
 +
a.hasrcorner = f;
 +
}
 +
var c = q.offset;
 +
var b = a.hasrcorner;
 +
if (b) {
 +
if (hide)
 +
b.style.visibility = "hidden";
 +
else {
 +
if (!a.offsetWidth)
 +
a.style.visibility = "inherit";
 +
a.style.top = (parseInt(a.style.top) + c) + "px";
 +
a.style.left = (parseInt(a.style.left) + c) + "px";
 +
b.style.width = (a.offsetWidth + (c * 2)) + "px";
 +
b.style.height = (a.offsetHeight + (c * 2)) + "px";
 +
var ft = 0;
 +
var fl = 0;
 +
if (qm_o) {
 +
ft = b[qp].clientTop;
 +
fl = b[qp].clientLeft;
 +
}
 +
if (qm_s2) {
 +
ft = qm_gcs(b[qp], "border-top-width", "borderTopWidth");
 +
fl = qm_gcs(b[qp], "border-left-width", "borderLeftWidth");
 +
}
 +
b.style.top = (a.offsetTop - c - ft) + "px";
 +
b.style.left = (a.offsetLeft - c - fl) + "px";
 +
b.style.visibility = "inherit";
 +
var s = b.childNodes;
 +
for (var i = 0; i < s.length; i++) {
 +
if (s[i].getAttribute("qmrcmid"))
 +
s[i].style.height = Math.abs((a.offsetHeight - (q.osize * 2) + (c * 2))) + "px";
 +
}
 +
}
 +
}
 +
};
 +
function qm_rcorner_get_span(size, i, top, tinset) {
 +
var q = qmad.rcorner;
 +
var mlmr;
 +
if (i == 0) {
 +
var mo = q.ps[size] + q.mid;
 +
if (q.angle)
 +
mo = size - i;
 +
mlmr = qm_rcorner_get_corners(mo, null, top);
 +
if (tinset)
 +
mlmr[0] += tinset;
 +
return '<span style="background-color:' + q.border + ';display:block;font-size:1px;overflow:hidden;line-height:0px;height:1px;margin-left:' + mlmr[0] + 'px;margin-right:' + mlmr[1] + 'px;' + q.opacity + '"></span>';
 +
} else {
 +
var md = size - (i);
 +
var ih = 1;
 +
var bs = 1;
 +
if (!q.angle) {
 +
if (i >= q.mid)
 +
ih = Math.abs(q.mid - i) + 1;
 +
else {
 +
bs = Math.abs(q.mid - i) + 1;
 +
md = q.ps[size - i] + q.mid;
 +
}
 +
if (top)
 +
q.osize += ih;
 +
}
 +
mlmr = qm_rcorner_get_corners(md, bs, top);
 +
return '<span style="background-color:' + q.background + ';border-color:' + q.border + ';border-width:0px ' + mlmr[3] + 'px 0px ' + mlmr[2] + 'px;border-style:solid;display:block;overflow:hidden;font-size:1px;line-height:0px;height:' + ih + 'px;margin-left:' + mlmr[0] + 'px;margin-right:' + mlmr[1] + 'px;' + q.opacity + '"></span>';
 +
}
 +
};
 +
function qm_rcorner_get_corners(mval, bval, top) {
 +
var q = qmad.rcorner;
 +
var ml = mval;
 +
var mr = mval;
 +
var bl = bval;
 +
var br = bval;
 +
if (top) {
 +
if (!q.corners[0]) {
 +
ml = 0;
 +
bl = 1;
 +
}
 +
if (!q.corners[1]) {
 +
mr = 0;
 +
br = 1;
 +
}
 +
} else {
 +
if (!q.corners[2]) {
 +
mr = 0;
 +
br = 1;
 +
}
 +
if (!q.corners[3]) {
 +
ml = 0;
 +
bl = 1;
 +
}
 +
}
 +
return new Array(ml, mr, bl, br);
 +
}/* ]]> */
 +
</script>
 +
 
 +
<!-- Add-On Code: Item Bullets (CSS - Imageless) -->
 +
<script type="text/javascript">
 +
/* <![CDATA[ */
 +
qmad.br_navigator = navigator.userAgent.indexOf("Netscape") + 1;
 +
qmad.br_version = parseFloat(navigator.vendorSub);
 +
qmad.br_oldnav6 = qmad.br_navigator &amp;&amp; qmad.br_version < 7;
 +
qmad.br_strict = ( dcm = document.compatMode) &amp;&amp; dcm == "CSS1Compat";
 +
qmad.br_ie = window.showHelp;
 +
qmad.str = (qmad.br_ie &amp;&amp; !qmad.br_strict);
 +
if (!qmad.br_oldnav6) {
 +
if (!qmad.ibcss)
 +
qmad.ibcss = new Object();
 +
if (qmad.bvis.indexOf("qm_ibcss_active(o,false);") == -1) {
 +
qmad.bvis += "qm_ibcss_active(o,false);";
 +
qmad.bhide += "qm_ibcss_active(a,1);";
 +
if (window.attachEvent)
 +
window.attachEvent("onload", qm_ibcss_init);
 +
else if (window.addEventListener)
 +
window.addEventListener("load", qm_ibcss_init, 1);
 +
if (window.attachEvent)
 +
document.attachEvent("onmouseover", qm_ibcss_hover_off);
 +
else if (window.addEventListener)
 +
document.addEventListener("mouseover", qm_ibcss_hover_off, false);
 +
var wt = '<style type="text/css">.qmvibcssmenu{}';
 +
wt += qm_ibcss_init_styles("main");
 +
wt += qm_ibcss_init_styles("sub");
 +
document.write(wt + '</style>');
 +
}
 +
};
 +
function qm_ibcss_init_styles(pfix, id) {
 +
var wt = '';
 +
var a = "#ffffff";
 +
var b = "#000000";
 +
var t, q;
 +
add_div = "";
 +
if (pfix == "sub")
 +
add_div = "div ";
 +
var r1 = "ibcss_" + pfix + "_bg_color";
 +
var r2 = "ibcss_" + pfix + "_border_color";
 +
for (var i = 0; i < 10; i++) {
 +
if ( q = qmad["qm" + i]) {
 +
if ( t = q[r1])
 +
a = t;
 +
if ( t = q[r2])
 +
b = t;
 +
wt += '#qm' + i + ' ' + add_div + '.qm-ibcss-static span{background-color:' + a + ';border-color:' + b + ';}';
 +
if ( t = q[r1 + "_hover"])
 +
a = t;
 +
if ( t = q[r2 + "_hover"])
 +
b = t;
 +
wt += '#qm' + i + '  ' + add_div + '.qm-ibcss-hover span{background-color:' + a + ';border-color:' + b + ';}';
 +
if ( t = q[r1 + "_active"])
 +
a = t;
 +
if ( t = q[r2 + "_active"])
 +
b = t;
 +
wt += '#qm' + i + '  ' + add_div + '.qm-ibcss-active span{background-color:' + a + ';border-color:' + b + ';}';
 +
}
 +
}
 +
return wt;
 +
};
 +
function qm_ibcss_init(e, spec) {
 +
var z;
 +
if (( z = window.qmv) &amp;&amp; ( z = z.addons) &amp;&amp; ( z = z.ibcss) &amp;&amp; (!z["on" + qmv.id] &amp;&amp; z["on" + qmv.id] != undefined &amp;&amp; z["on" + qmv.id] != null))
 +
return;
 +
qm_ts = 1;
 +
var q = qmad.ibcss;
 +
var a, b, r, sx, sy;
 +
z = window.qmv;
 +
for ( i = 0; i < 10; i++) {
 +
if (!( a = document.getElementById("qm" + i)) || (!isNaN(spec) &amp;&amp; spec != i))
 +
continue;
 +
var ss = qmad[a.id];
 +
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q.mtype = ss.ibcss_main_type;
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q.msize = ss.ibcss_main_size;
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if (!q.msize)
 +
q.msize = 5;
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q.md = ss.ibcss_main_direction;
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if (!q.md)
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md = "right";
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q.mbg = ss.ibcss_main_bg_color;
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q.mborder = ss.ibcss_main_border_color;
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sx = ss.ibcss_main_position_x;
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sy = ss.ibcss_main_position_y;
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if (!sx)
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sx = 0;
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sy = 0;
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q.mpos = eval("new Array('" + sx + "','" + sy + "')");
 +
q.malign = eval("new Array('" + ss.ibcss_main_align_x + "','" + ss.ibcss_main_align_y + "')");
 +
r = q.malign;
 +
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 +
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if (!r[1])
 +
r[1] = "center";
 +
q.stype = ss.ibcss_sub_type;
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q.ssize = ss.ibcss_sub_size;
 +
if (!q.ssize)
 +
q.ssize = 5;
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q.sd = ss.ibcss_sub_direction;
 +
if (!q.sd)
 +
sd = "right";
 +
q.sbg = ss.ibcss_sub_bg_color;
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q.sborder = ss.ibcss_sub_border_color;
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sx = ss.ibcss_sub_position_x;
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sy = ss.ibcss_sub_position_y;
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if (!sx)
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sx = 0;
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sy = 0;
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q.spos = eval("new Array('" + sx + "','" + sy + "')");
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q.salign = eval("new Array('" + ss.ibcss_sub_align_x + "','" + ss.ibcss_sub_align_y + "')");
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q.type = ss.ibcss_apply_to;
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qm_ibcss_create_inner("m");
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qm_ibcss_create_inner("s");
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qm_ibcss_init_items(a, 1, "qm" + i);
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};
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function qm_ibcss_create_inner(pfix) {
 +
var q = qmad.ibcss;
 +
var wt = "";
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var s = q[pfix + "size"];
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var type = q[pfix + "type"];
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var head;
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 +
gap = true;
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var v;
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 +
v = true;
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if (type.indexOf("arrow") + 1)
 +
type = "arrow";
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wt += qm_ibcss_get_span(s, i, pfix, type, null, null, v);
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if (head || gap)
 +
wt += qm_ibcss_get_span(s, null, pfix, null, head, gap, null);
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 +
var inner;
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if (type.indexOf("-inner") + 1)
 +
inner = true;
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var raised;
 +
if (type.indexOf("-raised") + 1)
 +
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type = "square";
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wt += qm_ibcss_get_span(s, i, pfix, type, null, null, null, inner, raised);
 +
if (inner)
 +
wt += qm_ibcss_get_span(s, i, pfix, "inner");
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}
 +
q[pfix + "inner"] = wt;
 +
};
 +
function qm_ibcss_get_span(size, i, pfix, type, head, gap, v, trans, raised) {
 +
var q = qmad.ibcss;
 +
var d = q[pfix + "d"];
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var it = i;
 +
var il = i;
 +
var ih = 1;
 +
var iw = 1;
 +
var ml = 0;
 +
var mr = 0;
 +
var bl = 0;
 +
var br = 0;
 +
var mt = 0;
 +
var mb = 0;
 +
var bt = 0;
 +
var bb = 0;
 +
var af = 0;
 +
var ag = 0;
 +
if (qmad.str) {
 +
af = 2;
 +
ag = 1;
 +
}
 +
var addc = "";
 +
if (v || trans)
 +
addc = "background-color:transparent;";
 +
if (type == "arrow") {
 +
if (d == "down" || d == "up") {
 +
if (d == "up")
 +
i = size - i - 1;
 +
bl = 1;
 +
br = 1;
 +
ml = i;
 +
mr = i;
 +
iw = ((size - i) * 2) - 2;
 +
il = -size;
 +
ih = 1;
 +
if (i == 0 &amp;&amp; !v) {
 +
bl = iw + 2;
 +
br = 0;
 +
ml = 0;
 +
mr = 0;
 +
iw = 0;
 +
if (qmad.str)
 +
iw = bl;
 +
} else {
 +
iw += af;
 +
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 +
} else if (d == "right" || d == "left") {
 +
if (d == "left")
 +
i = size - i - 1;
 +
bt = 1;
 +
bb = 1;
 +
mt = i;
 +
mb = i;
 +
iw = 1;
 +
it = -size;
 +
ih = ((size - i) * 2) - 2;
 +
if (i == 0 &amp;&amp; !v) {
 +
bt = ih + 2;
 +
bb = 0;
 +
mt = 0;
 +
mb = 0;
 +
ih = 0;
 +
} else
 +
ih += af;
 +
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 +
} else if (head || gap) {
 +
bt = 1;
 +
br = 1;
 +
bb = 1;
 +
bl = 1;
 +
mt = 0;
 +
mr = 0;
 +
mb = 0;
 +
ml = 0;
 +
var pp = 0;
 +
if (gap)
 +
pp = 2;
 +
var pp1 = 1;
 +
if (gap)
 +
pp1 = 0;
 +
if (d == "down" || d == "up") {
 +
iw = parseInt(size / 2);
 +
if (iw % 2)
 +
iw--;
 +
ih = iw + pp1;
 +
il = -(parseInt((iw + 2) / 2));
 +
if (head &amp;&amp; gap)
 +
ih += ag;
 +
else
 +
ih += af;
 +
iw += af;
 +
if (d == "down") {
 +
if (gap)
 +
pp++;
 +
it = -ih - pp + ag;
 +
bb = 0;
 +
} else {
 +
it = size - 1 + pp + ag;
 +
bt = 0;
 +
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 +
} else {
 +
ih = parseInt(size / 2);
 +
if (ih % 2)
 +
ih--;
 +
iw = ih + pp1;
 +
it = -(parseInt((iw + 2) / 2));
 +
if (head &amp;&amp; gap)
 +
iw += ag;
 +
else
 +
iw += af;
 +
ih += af;
 +
if (d == "right") {
 +
il = -ih - 1 - pp + ag;
 +
br = 0;
 +
} else {
 +
il = size - 1 + pp + ag;
 +
bl = 0;
 +
}
 +
}
 +
if (gap) {
 +
bt = 1;
 +
br = 1;
 +
bb = 1;
 +
bl = 1;
 +
}
 +
} else if (type == "square") {
 +
if (raised) {
 +
if (i == 2)
 +
return "";
 +
iw = size;
 +
ih = size;
 +
it = 0;
 +
il = 0;
 +
if (i == 0) {
 +
iw = 0;
 +
ih = size;
 +
br = size;
 +
it = 1;
 +
il = 1;
 +
if (qmad.str)
 +
iw = br;
 +
}
 +
} else {
 +
if (size % 2)
 +
size++;
 +
it = 1;
 +
ih = size;
 +
iw = size;
 +
bl = 1;
 +
br = 1;
 +
il = 0;
 +
iw += af;
 +
if (i == 0 || i == 2) {
 +
ml = 1;
 +
it = 0;
 +
ih = 1;
 +
bl = size;
 +
br = 0;
 +
iw = 0;
 +
if (qmad.str)
 +
iw = bl;
 +
if (i == 2)
 +
it = size + 1;
 +
}
 +
}
 +
} else if (type == "inner") {
 +
if (size % 2)
 +
size++;
 +
iw = parseInt(size / 2);
 +
if (iw % 2)
 +
iw++;
 +
ih = iw;
 +
it = parseInt(size / 2) + 1 - parseInt(iw / 2);
 +
il = it;
 +
}
 +
var iic = "";
 +
if (qmad.str)
 +
iic = "&nbsp;";
 +
return '<span style="' + addc + 'border-width:' + bt + 'px ' + br + 'px ' + bb + 'px ' + bl + 'px;border-style:solid;display:block;position:absolute;overflow:hidden;font-size:1px;line-height:0px;height:' + ih + 'px;margin:' + mt + 'px ' + mr + 'px ' + mb + 'px ' + ml + 'px;width:' + iw + 'px;top:' + it + 'px;left:' + il + 'px;">' + iic + '</span>';
 +
};
 +
function qm_ibcss_init_items(a, main) {
 +
var q = qmad.ibcss;
 +
var aa, pf;
 +
aa = a.childNodes;
 +
for (var j = 0; j < aa.length; j++) {
 +
if (aa[j].tagName == "A") {
 +
if (window.attachEvent)
 +
aa[j].attachEvent("onmouseover", qm_ibcss_hover);
 +
else if (window.addEventListener)
 +
aa[j].addEventListener("mouseover", qm_ibcss_hover, false);
 +
var skip = false;
 +
if (q.type != "all") {
 +
if (q.type == "parent" &amp;&amp; !aa[j].cdiv)
 +
skip = true;
 +
if (q.type == "non-parent" &amp;&amp; aa[j].cdiv)
 +
skip = true;
 +
}
 +
if (!skip) {
 +
if (main)
 +
pf = "m";
 +
else
 +
pf = "s";
 +
var ss = document.createElement("SPAN");
 +
ss.className = "qm-ibcss-static";
 +
var s1 = ss.style;
 +
s1.display = "block";
 +
s1.position = "relative";
 +
s1.fontSize = "1px";
 +
s1.lineHeight = "0px";
 +
s1.zIndex = 1;
 +
ss.ibhalign = q[pf+"align"][0];
 +
ss.ibvalign = q[pf+"align"][1];
 +
ss.ibposx = q[pf+"pos"][0];
 +
ss.ibposy = q[pf+"pos"][1];
 +
ss.ibsize = q[pf + "size"];
 +
qm_ibcss_position(aa[j], ss);
 +
ss.innerHTML = q[pf + "inner"];
 +
aa[j].qmibulletcss = aa[j].insertBefore(ss, aa[j].firstChild);
 +
ss.setAttribute("qmvbefore", 1);
 +
ss.setAttribute("isibulletcss", 1);
 +
if (aa[j].className.indexOf("qmactive") + 1)
 +
qm_ibcss_active(aa[j]);
 +
}
 +
if (aa[j].cdiv)
 +
new qm_ibcss_init_items(aa[j].cdiv, null);
 +
}
 +
}
 +
};
 +
function qm_ibcss_position(a, b) {
 +
if (b.ibhalign == "right")
 +
b.style.left = (a.offsetWidth + parseInt(b.ibposx) - b.ibsize) + "px";
 +
else if (b.ibhalign == "center")
 +
b.style.left = (parseInt(a.offsetWidth / 2) - parseInt(b.ibsize / 2) + parseInt(b.ibposx)) + "px";
 +
else
 +
b.style.left = b.ibposx + "px";
 +
if (b.ibvalign == "bottom")
 +
b.style.top = (a.offsetHeight + parseInt(b.ibposy) - b.ibsize) + "px";
 +
else if (b.ibvalign == "middle")
 +
b.style.top = parseInt((a.offsetHeight / 2) - parseInt(b.ibsize / 2) + parseInt(b.ibposy)) + "px";
 +
else
 +
b.style.top = b.ibposy + "px";
 +
};
 +
function qm_ibcss_hover(e, targ) {
 +
e = e || window.event;
 +
if (!targ) {
 +
var targ = e.srcElement || e.target;
 +
while (targ.tagName != "A")
 +
targ = targ[qp];
 +
}
 +
var ch = qmad.ibcss.lasth;
 +
if (ch &amp;&amp; ch != targ &amp;&amp; ch.qmibulletcss)
 +
qm_ibcss_hover_off(new Object(), ch);
 +
if (targ.className.indexOf("qmactive") + 1)
 +
return;
 +
var wo = targ.qmibulletcss;
 +
if (wo) {
 +
x2("qm-ibcss-hover", wo, 1);
 +
qmad.ibcss.lasth = targ;
 +
}
 +
if (e)
 +
qm_kille(e);
 +
};
 +
function qm_ibcss_hover_off(e, o) {
 +
if (!o)
 +
o = qmad.ibcss.lasth;
 +
if (o &amp;&amp; o.qmibulletcss)
 +
x2("qm-ibcss-hover", o.qmibulletcss);
 +
};
 +
function qm_ibcss_active(a, hide) {
 +
if (!hide &amp;&amp; a.className.indexOf("qmactive") == -1)
 +
return;
 +
if (hide &amp;&amp; a.idiv) {
 +
var o = a.idiv;
 +
if (o &amp;&amp; o.qmibulletcss) {
 +
x2("qm-ibcss-active", o.qmibulletcss);
 +
}
 +
} else {
 +
if (!a.cdiv.offsetWidth)
 +
a.cdiv.style.visibility = "inherit";
 +
qm_ibcss_wait_relative(a);
 +
var wo = a.qmibulletcss;
 +
if (wo)
 +
x2("qm-ibcss-active", wo, 1);
 +
}
 +
};
 +
function qm_ibcss_wait_relative(a) {
 +
if (!a)
 +
a = qmad.ibcss.cura;
 +
if (a.cdiv) {
 +
if (a.cdiv.qmtree &amp;&amp; a.cdiv.style.position != "relative") {
 +
qmad.ibcss.cura = a;
 +
setTimeout("qm_ibcss_wait_relative()", 10);
 +
return;
 +
}
 +
var aa = a.cdiv.childNodes;
 +
for (var i = 0; i < aa.length; i++) {
 +
if (aa[i].tagName == "A" &amp;&amp; aa[i].qmibulletcss)
 +
qm_ibcss_position(aa[i], aa[i].qmibulletcss);
 +
}
 +
}
 +
}/* ]]> */
 +
</script>
 +
 
 +
</head>
 +
 
 +
<body >
 +
 
 +
<div id="logospace"><IMG src="https://static.igem.org/mediawiki/2013/3/3e/Titech_title_logo.png" width="40%" border=0 ></div>
 +
 
 +
<ul id="qm0" class="qmmc">
 +
 
 +
<li>
 +
<a href="https://2013.igem.org/Team:Tokyo_Tech">Home</a>
 +
</li>
 +
<li>
 +
<a href="https://2013.igem.org/Team:Tokyo_Tech/Team">Team</a>
 +
</li>
 +
<li>
 +
<a class="qmparent" href="https://2013.igem.org/Team:Tokyo_Tech/Project">Projects</a>
 +
<ul>
 +
<li>
 +
<a href="javascript:void(0)">Zombie</a>
 +
</li>
 +
<li>
 +
<a href="javascript:void(0)">Plants</a>
 +
</li>
 +
<li>
 +
<a href="javascript:void(0)">Stands</a>
 +
</li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="https://2013.igem.org/Team:Tokyo_Tech/Attributions">Attributions</a>
 +
</li>
 +
<li>
 +
<a href="https://2013.igem.org/Team:Tokyo_Tech/Parts">Parts</a>
 +
</li>
 +
<li>
 +
<a href="javascript:void(0)">Human Practice</a>
 +
</li>
 +
 
 +
<li>
 +
<a class="qmparent" href="https://2013.igem.org/Team:Tokyo_Tech/Notebook">Notebook</a>
 +
<ul>
 +
<li>
 +
<a href="javascript:void(0)">Weekly Journal</a>
 +
</li>
 +
<li>
 +
<a href="javascript:void(0)">Protocols</a>
 +
</li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="https://2013.igem.org/Team:Tokyo_Tech/Safety">Safety</a>
 +
</li>
 +
<li>
 +
<a class="qmparent" href="javascript:void(0);">More</a>
 +
<ul>
 +
<li>
 +
<a href="https://igem.org/Team.cgi?year=2013&amp;team_name=Tokyo_Tech">Official Team Page</a>
 +
</li>
 +
<li>
 +
<a href="javascript:void(0);">Thanks</a>
 +
</li>
 +
</ul>
 +
</li>
 +
<li class="qmclear">
 +
&nbsp;
 +
</li>
 +
</ul>
 +
<script type="text/javascript">
 +
qm_create(0, false, 0, 500, false, false, false, false);
 +
</script>
 +
 
 +
</body>
 +
 
 +
 
 +
<body >
 +
<div class=textspace>
 +
<div class="#66FFFF">
 +
<h1>Abstract</h1><h2>Food shortages are said to be inevitable due to rapid population growth. Previous iGEM teams have approached to solve the problem by challenging to express plant hormone in E.Coli (2010 imperial college, 2012 Evry, 2012 Kyoto).
 +
<br>
 +
<br>
 +
Our goal is to make progress with transforming E. Coli to act upon plants. That is to say, we aim to create “Farmer E. Coli”, which sense the nutrition of soil and express the plant hormones properly. We need to synthesize Auxin, which is presented by Imperial College in 2010 iGEM, and Cytokinin on our own.
 +
<br>
 +
<br>
 +
We will find the solution of “Safety” and “Crosstalk” problems considered when we put this to practical use. As to safety, we plan to create E. Coli which destroys external E. Coli by genome transportation of phage. Furthermore, our new circuit design To avoid crosstalk, we will circumvent the crosstalk. </h2>
 +
</div>
 +
<table class="projecttable">
 +
<tr>
 +
<td align="center">
 +
<div class="blackboxmini"><IMG src="https://static.igem.org/mediawiki/2013/2/22/Titech_home_plant_logo.png" width="100%" height="auto" border=0 >
 +
</div></td>
 +
<td align="center">
 +
<div class="blackboxmini"><IMG src="https://static.igem.org/mediawiki/2013/c/c5/Titech_home_m13_logo.png" width="80%" height="auto"  border=0 >
 +
</div></td>
 +
<td align="center">
 +
<div class="blackboxmini"><IMG src="https://static.igem.org/mediawiki/2013/6/6f/Titech_home_crosstalk_logo.png" width="100%" height="auto" vspace="60%"  border=0 >
 +
</div></td>
 +
</tr>
 +
</table>
 +
</div>
 +
 
 +
</body>
 +
 
 +
</p>
 +
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 +
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Post-expand include size: 41/2097152 bytes
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Template argument size: 0/2097152 bytes
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Expensive parser function count: 0/100
 +
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<!-- Saved in parser cache with key 2013_igem_org:pcache:idhash:1544-0!1!0!!en!2!edit=0 and timestamp 20130813012621 -->
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<div class="printfooter">
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Retrieved from "<a href="https://2013.igem.org/Team:Tokyo_Tech">https://2013.igem.org/Team:Tokyo_Tech</a>"</div>
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      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
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                        <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Tokyo_Tech"
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Revision as of 03:02, 13 August 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Team:Tokyo Tech - 2013.igem.org

 

Team:Tokyo Tech

From 2013.igem.org

iGEM-2013 Titech

Abstract

Food shortages are said to be inevitable due to rapid population growth. Previous iGEM teams have approached to solve the problem by challenging to express plant hormone in E.Coli (2010 imperial college, 2012 Evry, 2012 Kyoto).

Our goal is to make progress with transforming E. Coli to act upon plants. That is to say, we aim to create “Farmer E. Coli”, which sense the nutrition of soil and express the plant hormones properly. We need to synthesize Auxin, which is presented by Imperial College in 2010 iGEM, and Cytokinin on our own.

We will find the solution of “Safety” and “Crosstalk” problems considered when we put this to practical use. As to safety, we plan to create E. Coli which destroys external E. Coli by genome transportation of phage. Furthermore, our new circuit design To avoid crosstalk, we will circumvent the crosstalk.

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