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UIUC Illinois/Safety/Database
From 2013.igem.org
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*Methyl Blue alternative- not nearly as hazardous, but may not be as effective as EtBr | *Methyl Blue alternative- not nearly as hazardous, but may not be as effective as EtBr | ||
==Solid Disposal== | ==Solid Disposal== | ||
| + | ===Sharps=== | ||
| + | * all sharps should be disposed of in a closed, labeled container. Nothing should ever be removed from the container and it must never overflow. | ||
| + | ** The following belong in sharps containers: medical needles, syringe barrels, pasteur pipettes, scalpel and razor blades, blood vials, microscope slides and coverslips, glassware contaminated with infectious agents | ||
| + | **do '''NOT''' put the following in sharps containers: plastic items, beverage containers, non-contaminated glassware, solvent/chemical bottles, light bulbs, paper materials, pipette tips, plastic pipettes, aerosol cans, scintillation vials, items with liquid (expect blood in vacutainers | ||
| + | ===Glassware=== | ||
| + | *glassware that has not been contaminated by biological, chemical, or radioactive materials can be disposed of in regular trash containers. For large amounts, place broken pieces in lined and labeled cardboard box. This box can then be disposed of in a dumpster. | ||
| + | **do not line the cardboard box with a biohazard or radiation hazard bag | ||
| + | ===Autoclavable biohazard bags=== | ||
| + | *used to collect any non-sharp items that have come into contact with biological materials. These bags must have the international biohazard symbol and be stored in a closed container also displaying the international biohazard symbol. After autoclaving, the bags must be placed in a opaque trash bags and sealed before being thrown away. | ||
| + | **examples of items: pipette tips, gloves, petri dishes, PCR tubes, microcentrifuge tubes, non-glass stereological pipette tips, inoculation tubes, falcon tubes | ||
| + | ***do not put liquids, glass, or sharps into biohazard bags | ||
| + | ===EtBr containers=== | ||
| + | *used to collect any materials that have been contaminated with Ethidium Bromide (EtBr). These containers are treated as hazardous waste and must be lined and have a lid. They must be clearly marked “EtBr Waster” and a Hazardous Waste Materials Pickup form must be filed in order to be picked up. | ||
| + | ** do not let the container overflow; the lid must keep all waste securely in the container | ||
| + | |||
==Liquid Disposal== | ==Liquid Disposal== | ||
==General decontamination== | ==General decontamination== | ||
Revision as of 15:19, 13 August 2013
Contents |
Safety Database
EtBr
[www.purdue.edu/rem/hmm/ethidbr.htm]
- Ethidium bromide(EtBR) is a known toxin and mutagen. Its use should be limited and it should be handled with EXTREME CARE.
- Only use EtBr in designated areas clearly marked “EtBr contamination” to avoid contaminating other lab areas and equipment.
- Any PPE that has been contaminated with EtBr must be disposed of in an EtBr container
- Latex gloves should not be worn when working with EtBr. Nitrile gloves are sufficient when minimal exposure time is expected. For longer periods of time, use multiple layers or chemically-resistant gloves.
- Wash hands thoroughly and immediately after disposing of gloves
- If accidental exposure occurs:
- Eyes- rinse with water for 15 minutes
- Skin- wash the contaminated area with soap and water
- Clothing- remove and place in sealed bag
- ALWAYS seek medical attention immediately after an accident has occurred
- Methyl Blue alternative- not nearly as hazardous, but may not be as effective as EtBr
Solid Disposal
Sharps
- all sharps should be disposed of in a closed, labeled container. Nothing should ever be removed from the container and it must never overflow.
- The following belong in sharps containers: medical needles, syringe barrels, pasteur pipettes, scalpel and razor blades, blood vials, microscope slides and coverslips, glassware contaminated with infectious agents
- do NOT put the following in sharps containers: plastic items, beverage containers, non-contaminated glassware, solvent/chemical bottles, light bulbs, paper materials, pipette tips, plastic pipettes, aerosol cans, scintillation vials, items with liquid (expect blood in vacutainers
Glassware
- glassware that has not been contaminated by biological, chemical, or radioactive materials can be disposed of in regular trash containers. For large amounts, place broken pieces in lined and labeled cardboard box. This box can then be disposed of in a dumpster.
- do not line the cardboard box with a biohazard or radiation hazard bag
Autoclavable biohazard bags
- used to collect any non-sharp items that have come into contact with biological materials. These bags must have the international biohazard symbol and be stored in a closed container also displaying the international biohazard symbol. After autoclaving, the bags must be placed in a opaque trash bags and sealed before being thrown away.
- examples of items: pipette tips, gloves, petri dishes, PCR tubes, microcentrifuge tubes, non-glass stereological pipette tips, inoculation tubes, falcon tubes
- do not put liquids, glass, or sharps into biohazard bags
- examples of items: pipette tips, gloves, petri dishes, PCR tubes, microcentrifuge tubes, non-glass stereological pipette tips, inoculation tubes, falcon tubes
EtBr containers
- used to collect any materials that have been contaminated with Ethidium Bromide (EtBr). These containers are treated as hazardous waste and must be lined and have a lid. They must be clearly marked “EtBr Waster” and a Hazardous Waste Materials Pickup form must be filed in order to be picked up.
- do not let the container overflow; the lid must keep all waste securely in the container
