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From 2013.igem.org

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Revision as of 09:46, 15 August 2013

Liquid Cultures, Miniprepped

• K592012 (Magenta) CAM

• RFP control CAM

• R0082 (OmpF promoter) CAM

• DH5α 3 (Tom and Adam's control) TET

• DH5α 5 (Tom and Adam's RBS + Cyan) TET

• DH5α 1 (Tom and Adam's RBS + cph8) TET

• S05058 (RBS + yellow) CAM

• K592010 (yellow) CAM

• K592018 (RBS + cph8) CAM

• K608002 (RBS + promoter) CAM

• B0015 (Terminator) CAM

• K322124 (RBS + cph8) CAM

( 3 replicates, #2, #3 and #4.)

NanoDropping Promoter + RBS Replicates

1 = 29.6ng/ul

1 = 29.2ng/ul

2 = 12.2ng/ul

2 = 12.5ng/ul

3 = 22.8ng/ul

3 = 23.6ng/ul

4 = 17.8ng/ul

4 = 17.5ng/ul

NanoDrop of liquid cultures

Liquid culture	NanoDrop result (ng/ul)
Magenta	106.9
RFP	42.9
OmpF	117.3
DH5α 3	46.3
S05058	67.1
Yellow	57.1
RBS + cph8	113.7
cph8 #4	49.2 and 48.8
cph8 #2	221.9 and 180.7
cph8 #3	70.8 and 70.8
Promoter and RBS	99.9
B0015	52.1
AMP plasmid	82.5

We sent off cph8 K322124 red light sensor for sequencing.

2 replicates each of #4, #3 and #2 from glycerol stocks were done. 10ul in Eppendorfs.