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Team:DTU-Denmark/Methods/PCR-touchUp
From 2013.igem.org
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== Example of the method of touch up PCR == | == Example of the method of touch up PCR == | ||
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For literature see([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313699/ link]) | For literature see([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313699/ link]) | ||
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Latest revision as of 16:57, 15 August 2013
PCR-touchUp
Example of the method of touch up PCR
Touch up PCR is a method of amplifying a PCR-product that have bad primers or are troublesome in other ways. This is an example:
- 98°C for 2:00 - Denaturing and warm up
- 98°C for 0:10 - Denaturing after each cycle
- 50°C for 0:30 - Annealing
- Temperature increment of 1°C/cycle
- 72°C for 2:00 - Elongation
- Go to number 2 - repeat 10 times
- Go to number 2 - repeat 5 times
- 72°C for 5:00 - Final extension
- End
For literature see([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313699/ link])
