Team:KAIST Korea

From 2013.igem.org

(Difference between revisions)
Line 986: Line 986:
<div clear='both'></div>
<div clear='both'></div>
-
<div style="border:2px solid #000000;padding:20px 20px 20px 20px;">
+
-
+
-
<span style="font-size:20px;font-weight:bold">Why does Autoregulation so necessary?</span></br></br>
+
-
<span id="little">When we bring external pathway into E.coli to mass-produce the protein, it may collide with E.coli’s innate pathways by consuming critical resources which generate the redox potential (ATP, NADH, or NADPH molecules). This means that E.coli has to reach certain reducing potential to perform exotic metabolic pathway in the cell. </span></br></br>
+
-
<span id="little">In conventional methods, the promoters controlling protein expression are repressed until the cells get capacity to produce the protein. Then, this promoter can be induced by putting inducer to the solution on proper cell phase; cell phase can be measured by O.D. value. This kind of method was used because we assume that cell cultural Optical Density (which synchronizes with cell number in the culture) represents inner-cell reduction potential. Although in many time this method successfully makes cells to induce, there are several downsides in this method. </span></br></br>
+
-
<span id="little">First of all, problem arises with the process of adding inducers like IPTG or arabinose into media. Because the induction time is decided only by considering cell density (O.D.value) not the capacity of the cells (reduction potential), products may not come out as we predicted. Also, it is an annoying job to measure optical density, finding optimal induction conditions. Even more, if we miss the right time point, we might have to throw them away and start from the first place...</span></br></br>
+
-
<span id="little">This is why we came up with our auto-regulative device. As the result of iGEM project this summer, we suggest an auto-regulation module free from induction which utilizes dual-phase switching system.</span></br>
+
-
+
-
</div>
+
-
+

Revision as of 12:23, 16 August 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Team:KAIST Korea/Project Background - 2013.igem.org

 

Team:KAIST Korea/Project Background

From 2012.igem.org

KAIST Korea 2013 iGEM

Project : Overview

Overview



What we see and handle in lab is mostly ‘microscopic’. But there are macroscopic stuffs out there! At night, we can see stars, and among them, we can find Lion’s heart, ‘Regulus’. Although it is not the brightest kind, Regulus still makes it easy to notice the Lion in the sky.

Like Regulus, we want our module to be helpful for others to find their own Lion in this universe of synthetic biology. Our module may not be magnificent as that of other teams but we don’t actually want to excel others. We rather want to facilitate the advance of synthetic biology. Like the heart which beats in autonomous manner, our module will run by itself. Regarding all these aspects, we call our project ‘Reguli’.



Kaist Footer

Retrieved from "https://2012.igem.org/Team:KAIST_Korea/Project_Background"

Retrieved from "http://2013.igem.org/Team:KAIST_Korea"