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From 2013.igem.org

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Revision as of 13:45, 19 August 2013

{ "date" : "2013-07-30", "author" : "thomas", "title" : "Bacillus transformation!!!", "content" : "In order to test the transformation protocol for B. subtilis that Groeningen team kindly sent to us I prepared a competent cell Media following this protocol. I proceeded then perfonming the transformation using the empty vector BBa_K823026. I finally plated the tranformed cells into LB agar plates with 10 ug/ml of Kanamycin. The day after took a picture of the plate and performed a Gram staining following this protocol.

As you can see from the pictures, seems that Bacillus was successful transformed!!!!", "tags" : "b. subtilis-pspac" }