"
Team:UNITN-Trento/Notebook/Labposts/08/16
From 2013.igem.org
(Difference between revisions)
Ggirelli (Talk | contribs)
(Created page with "{ "date" : "2013-08-07", "author" : "emil", "title" : "Test for integration in B. subtilis ", "content" : "<html>In order to demonstrate the integration of pXyl in Thr locus ...")
Newer edit →
(Created page with "{ "date" : "2013-08-07", "author" : "emil", "title" : "Test for integration in B. subtilis ", "content" : "<html>In order to demonstrate the integration of pXyl in Thr locus ...")
Newer edit →
Revision as of 13:51, 19 August 2013
{ "date" : "2013-08-07", "author" : "emil", "title" : "Test for integration in B. subtilis ", "content" : "In order to demonstrate the integration of pXyl in Thr locus (essential for the biosinthesis of threonine) I have prepared 40 ml of a minimal medium (MNGE) following the LMU munich protocol without the threonine.Then I did an inoculum from the plate obtained from the transformation of 29/7.
MNGE medium
| Compound | Amount |
|---|---|
| 10xMN-Medium | 3,68ml |
| Sterile water | 33,12ml |
| Glucose (20%) | 4ml |
| K-Glutamat (40%) | 200µl |
| Fe[III]-ammonium-citrate (2,2 mg/ml) | 200µl |
| Tryptophan (5 mg/ml) | 400µl |
| MgSO4 (1M) | 120µl |
| threonine (5 mg/ml)(in this case absent) | 400µl |
MN medium 10x 50 ml
",
"tags" : "B.subtilis-PXyl"
}
| Compound | Amount |
|---|---|
| K2HPO4 (x3 H2O) | 6,8 g |
| KH2PO4 | 3 g |
| Na-citrate (x 2 H2O) | 0.5 g |
