Team:UNITN-Trento/Notebook/Labposts/08/39

From 2013.igem.org

(Difference between revisions)
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"author" : "emil",
"author" : "emil",
"title" : "Emil's(re)begins",
"title" : "Emil's(re)begins",
-
"content" : "<html>I screened 5&micro;l of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the    results:</html>      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel image|<html> <img src=''/></html>}}<html>Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Promega-PCR-Gel'>purification protocol</a> with the following results:",
+
"content" : "<html>I screened 5&micro;l of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the    results:</html>      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel image|<html> <img src=''/></html>}}<html>Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Promega-PCR-Gel'>purification protocol</a> with the following results:</html>
 +
      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html>
 +
        <center>
 +
            <table>
 +
                <td>
 +
                  <th>Sample</th>
 +
                  <th>Quantities</th>
 +
                </td>
 +
                <td>
 +
                    <tr>GFP 1</tr>
 +
                    <tr>70.5 ng/6micro;l</tr>
 +
                </td>
 +
                <td>
 +
                    <tr>GFP 2</tr>
 +
                    <tr>52 ng/6micro;l</tr>
 +
                </td>
 +
                <td>
 +
                    <tr>PXyl 1</tr>
 +
                    <tr>110.4 ng/6micro;l</tr>
 +
                </td>
 +
                <td>
 +
                    <tr>Pxyl 2</tr>
 +
                    <tr>77.7 ng/6micro;l</tr>
 +
                </td>
 +
                <td>
 +
                    <tr>Pxyl 3</tr>
 +
                    <tr>137 ng/6micro;l</tr>
 +
                </td>
 +
            </table>
 +
          </center>
 +
        </html>}}
 +
    <html>After that I did a digestion overnight of 3 &micro;l of the GFP1 and the PXyl1 following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion'> digestion protocol</a>.</html>",
"tags" : "PXyl-GFP"
"tags" : "PXyl-GFP"
}
}

Revision as of 07:16, 20 August 2013

{ "date" : "2013-08-15", "author" : "emil", "title" : "Emil's(re)begins", "content" : "I screened 5µl of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the results:

gel image
Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the purification protocol with the following results: 

     
results

         

            GFP 170.5 ng/6micro;lGFP 252 ng/6micro;lPXyl 1110.4 ng/6micro;lPxyl 277.7 ng/6micro;lPxyl 3137 ng/6micro;l

                

                   Sample
                   Quantities
                
                
                    

                    

                
                

                    

                    

                
                 

                    

                    

                
                 

                    

                    

                
                 

                    

                    

                
             

           

        

   After that I did a digestion overnight of 3 µl of the GFP1 and the PXyl1 following the  digestion protocol.",

"tags" : "PXyl-GFP" }

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