"
Team:Kyoto/Notebook
From 2013.igem.org
(Difference between revisions)
(→LB Culture Medium) |
(→LB Culture Medium) |
||
| Line 67: | Line 67: | ||
|} | |} | ||
| - | 1.Measured reagents and put they in the Erlenmeyer flask | + | 1.Measured reagents and put they in the Erlenmeyer flask<br> |
| - | 2. | + | 2.<br> |
3.autoclaving | 3.autoclaving | ||
Revision as of 03:26, 21 August 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Material and method | Safety | Attributions |
|---|
Contents |
RNA Oscillator
Turing Pattern
Aug 20
Miniprep
| DNA | concentration[μg/μL] | 260/280 | 260/230 |
| 8/18 Ptet -1 | 220 | 1.69 | 1.87 |
| 8/18 Ptet -2 | 210 | 1.69 | 1.71 |
| 8/18 RBS-tetR-DT -1 | 236 | 1.63 | 1.69 |
| 8/18 RBS-tetR-DT -2 | 182 | 1.65 | 1.98 |
| 8/18 J23100-RBS-GFP-DT -1 | 334 | 1.71 | 2.12 |
| 8/18 J23100-RBS-luxR-DT -1 | 440 | 1.67 | 1.60 |
| 8/18 J23100-RBS-luxR-DT -2 | 344 | 1.68 | 1.83 |
| 8/18 J23100-RBS-lacZα-DT -1 | 280 | 1.70 | 1.81 |
| 8/18 RBS-lysis3 -1 | 282 | 1.67 | 1.76 |
| 8/18 RBS-lysis3 -2 | 212 | 1.30 | 1.35 |
LB Culture Medium
volume - 200mL
| name | amount |
| Bacto Trypton | 2g |
| Bacto yeast extract | 1g |
| Nacl | 1g |
| Agar Powder | 2g |
| Amp | 40μl |
1.Measured reagents and put they in the Erlenmeyer flask
2.
3.autoclaving
not written yet
