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Revision as of 03:26, 21 August 2013 (view source) Ｙｔ (Talk | contribs) (→LB Culture Medium) ← Older edit		Revision as of 03:50, 21 August 2013 (view source) Mitsuaki (Talk | contribs) (→Ristriction Enzyme Proccessing) Newer edit →
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	====Ristriction Enzyme Proccessing====		====Ristriction Enzyme Proccessing====
		+	{|class="wikitable"
		+	|8/20 Ptet -1(220μg/mL)||EcoRI||XbaI||10x BSA||10x buffer M||MilliQ||total
		+	|-
		+	|4.5||1||1||3||3||17.5||30
		+	|-
		+	|0.5||0.2||0||1||1||7.3||10
		+	|-
		+	|0.5||0||0.2||1||1||7.3||10
		+	|-
		+	|0.5||0||0||1||1||7.5||10
		+	|}
		+	{|class="wikitable"
		+	|8/20 RBS-tetR-DT -2(182μg/mL)||EcoRI||SpeI||10x buffer H||MilliQ||total
		+	|-
		+	|5.5||1||1||3||19.5||30
		+	|-
		+	|0.6||0.2||0||1||8.2||10
		+	|-
		+	|0.6||0||0.2||1||8.2||10
		+	|-
		+	|0.6||0||0||1||8.4||10
		+	|}
		+
	====Transformation====		====Transformation====
	====Electrophoresis====		====Electrophoresis====

---

Revision as of 03:50, 21 August 2013

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""m&m""

Aug 20

Miniprep

DNA	concentration[μg/μL]	260/280	260/230
8/18 Ptet -1	220	1.69	1.87
8/18 Ptet -2	210	1.69	1.71
8/18 RBS-tetR-DT -1	236	1.63	1.69
8/18 RBS-tetR-DT -2	182	1.65	1.98
8/18 J23100-RBS-GFP-DT -1	334	1.71	2.12
8/18 J23100-RBS-luxR-DT -1	440	1.67	1.60
8/18 J23100-RBS-luxR-DT -2	344	1.68	1.83
8/18 J23100-RBS-lacZα-DT -1	280	1.70	1.81
8/18 RBS-lysis3 -1	282	1.67	1.76
8/18 RBS-lysis3 -2	212	1.30	1.35

LB Culture Medium

volume - 200mL

name	amount
Bacto Trypton	2g
Bacto yeast extract	1g
Nacl	1g
Agar Powder	2g
Amp	40μl

1.Measured reagents and put they in the Erlenmeyer flask
2.
3.autoclaving

not written yet

Ristriction Enzyme Proccessing

8/20 Ptet -1(220μg/mL)	EcoRI	XbaI	10x BSA	10x buffer M	MilliQ	total
4.5	1	1	3	3	17.5	30
0.5	0.2	0	1	1	7.3	10
0.5	0	0.2	1	1	7.3	10
0.5	0	0	1	1	7.5	10

8/20 RBS-tetR-DT -2(182μg/mL)	EcoRI	SpeI	10x buffer H	MilliQ	total
5.5	1	1	3	19.5	30
0.6	0.2	0	1	8.2	10
0.6	0	0.2	1	8.2	10
0.6	0	0	1	8.4	10

Transformation

Electrophoresis