Team:Wisconsin-Madison/protocol

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Protocol

Expression and Purification of Enzymes

Solutions

Resuspension Buffer

  • 400 mM NaCl
  • 50 mM NaHPO4
  • 2.5% (v/v) glycerol
  • 15 mM Imidazole
  • 0.05% Triton X-100
  • pH 8.0

  • Retrieved from "http://2013.igem.org/Team:Wisconsin-Madison/protocol"