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Team:DTU-Denmark/Notebook/7 June 2013
From 2013.igem.org
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*prepare solutions | *prepare solutions | ||
*[[Team:DTU-Denmark/Methods/Autoclaving|autoclaving]] | *[[Team:DTU-Denmark/Methods/Autoclaving|autoclaving]] | ||
| - | *plate E.coli | + | *plate ''E.coli'' |
==Who was in the lab== | ==Who was in the lab== | ||
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===plating E.coli=== | ===plating E.coli=== | ||
| - | *Colonies of Top 10 E.coli were streaked on two plates | + | *Colonies of Top 10 ''E.coli'' were streaked on two plates |
*Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan) | *Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan) | ||
Revision as of 10:19, 22 August 2013
07 June 2013
Contents |
lab 208
Main purposes today
- helloworld-project
- prepare solutions
- autoclaving
- plate E.coli
Who was in the lab
Kristian
Procedure
- 1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
- 50% glycerol (500 ml)
- LB medium (liquid) (3 L);
->20 g of LB in every 1 L of distilled water. Autoclaved medium
->in 1 of these L, 14 g of agar were added for making LB solid medium
plating E.coli
- Colonies of Top 10 E.coli were streaked on two plates
- Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)
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