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Team:Grenoble-EMSE-LSU/Documentation/Notebook/August
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<h1>August</h1> | <h1>August</h1> | ||
| + | <h2>Week 1</h2> | ||
| + | <h2>Week 2</h2> | ||
| + | <h2>Week 3</h2> | ||
| + | <h3>Monday</h3> | ||
| + | <h3>Tuesday</h3> | ||
| + | <h3>Wedsnesday</h3> | ||
| + | <h3>Thursday</h3> | ||
| + | <h4> KillerRed Characterization </h4> | ||
| + | <p> Tested different loading dyes enabling to follow cell viability by fluorescence acquisition. The ON culture of M15[pRep4-pQE30::KR] was split in two different Erlenmeyers and incubated at 37°C, 300rpm. One of them was kept in the dark while the other was illuminated (I = ) for 2 hours to induce cell death. For both samples, 200 µL were pipetted, centrifuged, and incubated 15 min (37°C, 200 rpm) with 200µL of one of the following loading dyes : 5-100µM Diaminofluorescein, 500-5nM MitoTracker Green FM (M7514, Invitrogen, Carlsbad, NM, USA), XX SYBR Safe DNA gel stain (S33102, Invitrogen). Cells were subsequently washed in 200 µL PBS, mounted on a microscope slide and observed by fluorescence microscopy (FITC filter cube). | ||
| + | <h3>Friday</h3> | ||
| + | |||
| + | <h2>Week 4</h2> | ||
| + | |||
<p></p> | <p></p> | ||
</li> | </li> | ||
Revision as of 14:09, 22 August 2013
