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| | <script src="https://googledrive.com/host/0B4ZBZOYYKBzEZTdBSFdUV19LYjQ" type="text/javascript"></script> | | <script src="https://googledrive.com/host/0B4ZBZOYYKBzEZTdBSFdUV19LYjQ" type="text/javascript"></script> |
| | <script src="https://googledrive.com/host/0B4ZBZOYYKBzEblVWdXkta245Y0k/" type="text/javascript"> </script> <!--debounce--> | | <script src="https://googledrive.com/host/0B4ZBZOYYKBzEblVWdXkta245Y0k/" type="text/javascript"> </script> <!--debounce--> |
| - |
| |
| | <style> | | <style> |
| | | | |
| | /*Page specific*/ | | /*Page specific*/ |
| | + | |
| | + | /*format the figures. Each individual image may be floated left or right or given a margin |
| | + | in the html. Add this class to an image to give it a decent size and margins*/ |
| | + | |
| | .figure { | | .figure { |
| | display: inline-block; | | display: inline-block; |
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| | margin-right:20px; | | margin-right:20px; |
| | margin-bottom: 10px; | | margin-bottom: 10px; |
| - |
| + | } |
| - | }
| + | |
| - |
| + | /*format title (center)*/ |
| - | .left_wrap {
| + | |
| - | min-height: 1975px;
| + | |
| - | }
| + | |
| - |
| + | |
| - | | + | |
| | h2 { | | h2 { |
| | text-align: center; | | text-align: center; |
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| | font-size: 18px; | | font-size: 18px; |
| | color: black; | | color: black; |
| - | } | + | } |
| - |
| + | |
| - | .min-margin {
| + | |
| - | width: 200px;
| + | |
| - | height: 2000px;
| + | |
| - | }
| + | |
| - |
| + | |
| - | .section-title {
| + | |
| - | font-family: arial, sans-serif;
| + | |
| - | font-size: 25px;
| + | |
| - | -webkit-transform: rotate(-90deg);
| + | |
| - | -moz-transform: rotate(-90deg);
| + | |
| - | -o-transform: rotate(-90deg);
| + | |
| - | -ms-transform: rotate(-90deg);
| + | |
| - | transform: rotate(-90deg);
| + | |
| - | }
| + | |
| - |
| + | |
| - |
| + | |
| - |
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| - |
| + | |
| - |
| + | |
| | </style> | | </style> |
| | </head> | | </head> |
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| | | | |
| | | | |
| - | <!-- <div class="logo-wrap"><img src="https://googledrive.com/host/0B4ZBZOYYKBzEUlI3ZDU2OGRrc1E" id="penn"/></div><!--penn logo-->
| + | |
| - |
| + | <!--*************** |
| | + | Sidebar |
| | + | **************--> |
| | <ul class="navbar"> | | <ul class="navbar"> |
| - | <a href="https://2013.igem.org/Team:Penn"><li>HOME</li></a> | + | <a href="https://2013.igem.org/Team:Penn"><li>home</li></a> |
| | <li class="dropdown" id="project"> | | <li class="dropdown" id="project"> |
| - | <a href="#" class="active" data-toggle="dropdown"> PROJECT<div class="arrow">></div> <b class="caret"></b></a> | + | <a href="#" class="active" data-toggle="dropdown"> project<div class="arrow">></div> <b class="caret"></b></a> |
| | <ul class="dropdown-menu" id="drop-menu1"> | | <ul class="dropdown-menu" id="drop-menu1"> |
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| | </li> | | </li> |
| | <li class="dropdown" id="about"> | | <li class="dropdown" id="about"> |
| - | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> ABOUT<div class="arrow">></div> <b class="caret"></b></a> | + | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> about<div class="arrow">></div> <b class="caret"></b></a> |
| | <ul class="dropdown-menu" id="drop-menu2"> | | <ul class="dropdown-menu" id="drop-menu2"> |
| | <a href="https://2013.igem.org/Team:Penn/Team"> <li>team</li></a> | | <a href="https://2013.igem.org/Team:Penn/Team"> <li>team</li></a> |
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| | </li></ul> | | </li></ul> |
| | | | |
| - | <a href="https://2013.igem.org/Team:Penn/HumanPractices"><li>HUMAN PRACTICES</li></a> | + | <a href="https://2013.igem.org/Team:Penn/HumanPractices"><li>human practices</li></a> |
| - | <a href="https://2013.igem.org/Team:Penn/Notebook"><li>NOTEBOOK</li></a> | + | <a href="https://2013.igem.org/Team:Penn/Notebook"><li>notebook</li></a> |
| | </ul> | | </ul> |
| | | | |
| | | | |
| - | <div class="section-title" style=" margin-top: 150px; position: relative;">abstract</div> | + | <!---**section titles--if you add more sections, change the margin of the new one to be further down**--> |
| - | <div class="section-title" style=" margin-top: 400px; position: relative;">methods</div>
| + | <div class="section-title" style=""> project</div> |
| - | <div class="section-title" style=" margin-top: 750px; position: relative;"> results</div> | + | |
| | | | |
| | | | |
| | </div> | | </div> |
| - | <div class="section1"> | + | <div class="section1"><!--first section--> |
| - | <div class="text"> | + | <div class="text"><!--white text box--> |
| - | <h2>ABSTRACT</h2> | + | <h2>ABSTRACT</h2> <!--title--> |
| | <p>The code of life is much more than a sequence of A's, G's, C's and T's; | | <p>The code of life is much more than a sequence of A's, G's, C's and T's; |
| | a suite of epigenetic mechanisms, ranging from chromatin remodeling to non-coding RNAs, | | a suite of epigenetic mechanisms, ranging from chromatin remodeling to non-coding RNAs, |
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| | </div> | | </div> |
| | | | |
| - | </div>
| + | |
| - |
| + | |
| - |
| + | |
| - | <div class="section2">
| + | |
| | <div class="text" id="methods"> | | <div class="text" id="methods"> |
| | + | <p><!--content--></p> |
| | </div> | | </div> |
| | <div class="text" id="methods"> | | <div class="text" id="methods"> |
| | + | <p><!--content--></p> |
| | </div> | | </div> |
| | <div class="text" id="methods"> | | <div class="text" id="methods"> |
| | + | <p><!--content--></p> |
| | </div> | | </div> |
| | <div class="text" id="methods"> | | <div class="text" id="methods"> |
| | + | <p><!--content--></p> |
| | </div> | | </div> |
| | <div class="text" id="methods"> | | <div class="text" id="methods"> |
| | + | <p><!--content--></p> |
| | </div> | | </div> |
| - | </div>
| + | |
| | | | |
| - | <div class="section3">
| + | |
| | | | |
| - |
| + | |
| - | <div class="text-wrap"> | + | <!--text-wrap: use this to wrap two or more text boxes as long as the width plus margins |
| | + | of the content doesn't equal more than 700. Float one left, one right to have two columns |
| | + | (for more than 2, add margins to center it. The text-wrap keeps the margins even between |
| | + | the sidebar and the rest of the page--> |
| | + | <div class="text-wrap"> |
| | | | |
| | | | |
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| | </div> | | </div> |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| | + | |
| | | | |
| | </body> | | </body> |
| | | | |
| | </html> | | </html> |
ABSTRACT
The code of life is much more than a sequence of A's, G's, C's and T's;
a suite of epigenetic mechanisms, ranging from chromatin remodeling to non-coding RNAs,
affect gene expression and cellular function.
In particular, DNA methylation has been
shown to alter transcriptional activity in a powerful, heritable manner. Abnormal methylation
patterns are associated with diseases including immunodeficiency syndromes, neurodevelopmental
disorders, and many types of cancer. Comprehensive understanding and control of DNA methylation
could be invaluable to researchers studying these diseases.
Synthetic biologists and geneticists are accustomed to turning genes on and off at will,
but the tools don’t exist to easily manipulate epigenetic patterns. We are developing a novel
fusion protein that enables site-specific methylation, which can repress promoter activity with
high precision.
In coming years, this fusion protein could become a powerful tool for epigenetics
researchers looking to perform on/off studies in the vein of classical genetics, as well as an
orthogonal mode of repressing constitutive promoters for bacterial synthetic biologists.
Eventually, it could even give clinical researchers the means to restore healthy methylation
levels in many insofar-untreatable epigenetic diseases.
"
