Team:DTU-Denmark/Methods/dpni treatment

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Revision as of 10:39, 23 August 2013

DpnI treatment

To digest methylated DNA, for example to clean up PCR products from template.

Reaction Mix:

  • 44 uL sample
  • 5 uL NEB buffer 4
  • 1 uL DpnI

Incubate 1h at 37C and 20:00 at 80C to inactivate the enzyme.

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