23/08/13

(Difference between revisions)

Revision as of 13:57, 23 August 2013

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Table representing the concentrations of herring sperm DNA before and after different treatments

Sample	Concentration
Sheared/Unsonicated (s.u.s)	17900ng/ul=17.9mg/ml
S.u.s 10fold dil.	1790.1ng/ul
S.u.s 100fold dil	152.6ng/ul
Sheared/Sonicated (s.s)	17667ng/ul=17.6mg/ml
S.s 10fold dil.	1766.7ng/ul
S.s 100fold dil.	156.2ng/ul
Sheared/sonicated overnight	Concentration in the tube=Average of the two samples=16.9mg/ml
500ul(DNA) in 500ul(1xTE)	14485.9ng/ul
2ml(DNA) in 10ml(1xTE)	3883ng/ul

Concentrations decrease with dilutions
The stock concentration in sheared/sonicated tube varies slightly, when derived from measured dilution concentrations that were subjected to different treatments (i.e. overnight incubation), though they orignated from the same tube
Difference in concentration is not too significant though: proposed 17.6mg/ml vs 16.9mg/ml

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+<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;">
+{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
+!align="center"|[[Team:Leicester|Home]]
+!align="center"|[[Team:Leicester/Team|Team]]
+!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
+!align="center"|[[Team:Leicester/Project|Project]]
+!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
+!align="center"|[[Team:Leicester/Modeling|Modeling]]
+!align="center"|[[Team:Leicester/Notebook|Notebook]]
+!align="center"|[[Team:Leicester/Safety|Safety]]
+!align="center"|[[Team:Leicester/Attributions|Attributions]]
+|}
+</div>
 ==Nanodropping overnight incubations of herring sperm DNA==
 *Table representing the concentrations of herring sperm DNA before and after different treatments