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From 2013.igem.org

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	July 9th		July 9th
-	1.	+	1. Performed NaCl Growth Curve Assay on E.coli containing PprA and RecA.
		+	2. Ran gels of PCR products.
		+	3. Performed Miniprep.
		+	4. Performed Survival Growth Assay.
		+
		+	July 10th
		+	1. Counted colonies on plates from yesterday's Survival Growth Assay.
		+	2. Performed PCR
		+	3. Performed Miniprep.
		+
		+	July 15th
		+	1. Ran gel of of 7/9/13 and 7/10/13 Miniprep products.
		+	2. Ran gel of PCR products from 7/10/13
		+	3. Presentation on Restriction Digest protocol and created table for 7/16/13 digest.
		+
		+	July 16th
		+	1. Performed Restriction Digest according to iGEM's restriction digest protocol.
		+	2. Reviewed iGEM's ligation protocol and created table for 7/17/13.
		+
		+	July 17th
		+	1. Ran gel of digest products to ensure digest was successful.
		+	2. Performed ligation according to iGEM's ligation protocol.
		+
		+	July 18th
		+	1. Team split up to work on transformation and DNA isolation.
		+
		+	July 19th
		+	1. First round of wiki assignments due.

---

Revision as of 02:17, 26 August 2013

• Home
• Project
• Team
• Notebook
• Attributions

Calendar

Daily Logs

May 20th

 First iGEM meeting of the summer!
    1. Developed goals for the summer
    2. Reviewed lab techniques
    3. Reviewed iGEM website and registry
    4. Assigned biobricks of interest and genes of interest to present

May 21st

    1. Members began their biobrick presentations.
    2. We covered online resources available to the team such as Open Wetware, iGEM Help pages, and Rstudio. 
    3. Team developed protocol for our Growth Curve Assay.

May 22nd

    1. Members continued biobrick and gene presentations.
    2. Team split into 5 groups and performed our first Growth Curve Assay.  

May 28th

    1. Biobrick and gene presentations continued. 
    2. Reviewed protocol for Polymerase Chain Reaction.

May 29th

    1. Biobrick and gene presentations continued.
    2. Team covered requirements for designing primers, determined primers for all genes of interest.

June 4th

    1. Reviewed protocol for DNA isolation. 
    2. Biotech student Beau came in to present his project on Deinococcus radiodurans.

June 5th

    1. Performed Hydrogen Peroxide Growth Curve Assay to determine appropriate concentration of hydrogen peroxide.

June 6th

    1. Performed NaCl Growth Curve Assay to determine appropriate concentration of NaCl. 
    2. Planned next stages of our project. 

June 10th

    1. Team presented primer designs.

June 11th

    1. Performed Hydrogen Peroxide Growth Curve Assay with different concentrations of hydrogen peroxide. 
    2. Team searched for homolog genes of interest in D. hopiensis. 

June 12th

    1. Team double checked primer designs for restriction sites Xba1, Pst1, Ecor1, and Spe1.

June 13th

    1. Performed Hydrogen Peroxide Growth Curve Assay, still searching working with different concentrations of hydrogen peroxide. 
    2. Presentation on iGEM's transformation protocol.

June 14th

    1. Team went to ASU for meet-and-greet luncheon with ASU's 2013 iGEM team. 

June 17th

    1. De-briefed members unable to attend ASU luncheon.
    2. Fern and Cristina debuted first clips of our team stop motion video!

June 18th

    1. Added prefix and suffix sequences to primers, reviewed entire sequence once more prior to ordering.
    2. Presentation on Alkaline Lysis Plasmid Miniprep protocol, drew up flowchart. 

June 19th

    1. Presentation on Gel Electrophoresis protocol. 

June 20th

    1. Performed Miniprep and Gel Electrophoresis.

June 25th

    1. Performed Miniprep. 

June 26th

    1. Ran yesterday's miniprep products out on a gel. 
    2. Performed NaCl Growth Curve Assay. 
    3. Developed protocol and flowchart for Survival Growth Assay. 

June 27th

    1. Performed NaCl Growth Curve Assay, working with higher concentration of IPTG. 
    2. Performed Survival Growth Assay. 

July 1st

    1. Presentation on adding information and navigating our wiki page. 
    2. First round of wiki sections assigned. 

July 2nd

    1. Team split up and performed NaCl Growth Curve Assay on transformed E.coli containing PprI gene or RecA gene (depending on group). 
    2. Performed Survival Growth Assay. 

July 3rd

    1. Performed transformation on LacI promoter (BBa_R0010), Ribosome Binding Site (BBa_B0034), Double Terminator (BBa_B0015), and RFP Control provided by iGEM.

July 8th

    1. Set up bacterial growth culture of transformation products from 7/8/13.
    2. Performed Polymerase Chain Reaction. 

July 9th

    1. Performed NaCl Growth Curve Assay on E.coli containing PprA and RecA. 
    2. Ran gels of PCR products. 
    3. Performed Miniprep. 
    4. Performed Survival Growth Assay. 

July 10th

    1. Counted colonies on plates from yesterday's Survival Growth Assay. 
    2. Performed PCR
    3. Performed Miniprep. 

July 15th

    1. Ran gel of of 7/9/13 and 7/10/13 Miniprep products. 
    2. Ran gel of PCR products from 7/10/13
    3. Presentation on Restriction Digest protocol and created table for 7/16/13 digest.

July 16th

    1. Performed Restriction Digest according to iGEM's restriction digest protocol.
    2. Reviewed iGEM's ligation protocol and created table for 7/17/13.

July 17th

    1. Ran gel of digest products to ensure digest was successful. 
    2. Performed ligation according to iGEM's ligation protocol.

July 18th

    1. Team split up to work on transformation and DNA isolation. 

July 19th

    1. First round of wiki assignments due.