Team:Groningen/Labwork/26 August 2013

From 2013.igem.org

(Difference between revisions)
Line 24: Line 24:
Plates (S7, S8, S11, S12, S13, S14) showed roughly hundreds of colonies per plate. One colony per plate is tested using ColonyPCR with VF2 and VR primers.
Plates (S7, S8, S11, S12, S13, S14) showed roughly hundreds of colonies per plate. One colony per plate is tested using ColonyPCR with VF2 and VR primers.
<br><img src="https://static.igem.org/mediawiki/2013/7/7e/ColonyPCR-IDTgenes26082013.jpg" width="50%"></img>
<br><img src="https://static.igem.org/mediawiki/2013/7/7e/ColonyPCR-IDTgenes26082013.jpg" width="50%"></img>
 +
<br>The colonies contain the right construct.
<br><br>Plates (S3, S4, S9 and S10) showed 1-2 colonies per plate. One colony per plate is inoculated in LB + Amp (<b>Sander</b>).
<br><br>Plates (S3, S4, S9 and S10) showed 1-2 colonies per plate. One colony per plate is inoculated in LB + Amp (<b>Sander</b>).
<br><br>Plates (S15 and S16) showed no colonies.
<br><br>Plates (S15 and S16) showed no colonies.

Revision as of 18:40, 26 August 2013

Claudio

Plates (S7, S8, S11, S12, S13, S14) showed roughly hundreds of colonies per plate. One colony per plate is tested using ColonyPCR with VF2 and VR primers.

The colonies contain the right construct.

Plates (S3, S4, S9 and S10) showed 1-2 colonies per plate. One colony per plate is inoculated in LB + Amp (Sander).

Plates (S15 and S16) showed no colonies.