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Team:Paris Saclay/Notebook/July/4
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{{Team:Paris_Saclay/incl_debut_generique}} | {{Team:Paris_Saclay/incl_debut_generique}} | ||
='''Notebook : July 4'''= | ='''Notebook : July 4'''= | ||
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| + | ===''Summary: ''=== | ||
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| + | *calculated the size of segments for bands of electrophoresis by using Clone Manager. | ||
| + | *Made interpretation for electrophoresis. The picture shows that the experimental result was coherent with our estimation. We ensure that we had our first BrioBrick, BBa_K1155000 (fnr+plasmid PSB1C3). | ||
| + | *stored 2 colonies who contain BioBrick BBa_K1155000 | ||
| + | *As the Bi.oBrick BBa_k1155000 was confirmed, the plasmid DNA extraction was performed | ||
| + | *Received the bacterial strain: pseudomonas KE707. | ||
| + | *The extract of plasmid DNA which contain BBa_K1155000 was digested by Not I, Mlu I, Hpa I, and were amplified by PCR. | ||
| + | *Seeded the 4 additional broths (2 for amilCP, 2 for FNR) for plasmid extraction). | ||
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=='''Lab work'''== | =='''Lab work'''== | ||
Revision as of 02:33, 28 August 2013
Notebook : July 4
Summary:
- calculated the size of segments for bands of electrophoresis by using Clone Manager.
- Made interpretation for electrophoresis. The picture shows that the experimental result was coherent with our estimation. We ensure that we had our first BrioBrick, BBa_K1155000 (fnr+plasmid PSB1C3).
- stored 2 colonies who contain BioBrick BBa_K1155000
- As the Bi.oBrick BBa_k1155000 was confirmed, the plasmid DNA extraction was performed
- Received the bacterial strain: pseudomonas KE707.
- The extract of plasmid DNA which contain BBa_K1155000 was digested by Not I, Mlu I, Hpa I, and were amplified by PCR.
- Seeded the 4 additional broths (2 for amilCP, 2 for FNR) for plasmid extraction).
Lab work
constructing
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