Team:Yale/Project Bioassay

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!align="center" style="background-color:#FFFFFF;" cellpadding="0" cellspacing="0" border="0"|<html><a href="https://2013.igem.org/Team:Yale/Project_Validate"><img src="https://encrypted-tbn3.gstatic.com/images?q=tbn:ANd9GcTVb8adyUdr2p_7aBWNSMfB83hCbKIaQhdkJ_k21JZ4PXnle8w8" style="-moz-transform: scaleX(-1); -webkit-transform: scaleX(-1); -o-transform: scaleX(-1); transform: scaleX(-1); -ms-filter: fliph; /*IE*/ filter: fliph; /*IE*/" width="50" height="50" title="Previous: Validate PLA synthesis" /></a></html>
!align="center"|[[Team:Yale/Project_Overview|Project Overview]]
!align="center"|[[Team:Yale/Project_Overview|Project Overview]]
!align="center"|[[Team:Yale/Project_Validate|Validate PLA synthesis]]
!align="center"|[[Team:Yale/Project_Validate|Validate PLA synthesis]]
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!align="center"|[[Team:Yale/Project_Export|Introduce export system]]
!align="center"|[[Team:Yale/Project_Export|Introduce export system]]
!align="center"|[[Team:Yale/Project_Bioplastic|Make a bioplastic]]
!align="center"|[[Team:Yale/Project_Bioplastic|Make a bioplastic]]
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!align="center" style="background-color:#FFFFFF;" cellpadding="0" cellspacing="0" border="0"|<html><a href="https://2013.igem.org/Team:Yale/Project_MAGE"><img src="https://encrypted-tbn3.gstatic.com/images?q=tbn:ANd9GcTVb8adyUdr2p_7aBWNSMfB83hCbKIaQhdkJ_k21JZ4PXnle8w8" width="50" height="50" title="Next: Apply MAGE" /></a></html>
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Revision as of 04:12, 28 August 2013

Project Overview Validate PLA synthesis Develop bioassay Apply MAGE Introduce export system Make a bioplastic


Aims for the Project

  1. Engineer strains of E. coli to validate PLA synthesis
  2. Develop bioassay to screen PLA production
  3. Apply MAGE to optimize PLA production, guided by FBA
  4. Introduce type 1 secretion system to export and extract PLA
  5. Make a bioplastic


Develop bioassay to screen PLA production

Nile red PHB stain.jpg

FACS.jpg