Georgia State/15 May 2013

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5/13 Group B: Made LB Broth and overnight cultures of pGAPzα B Group C: Made YPD broth;<br /> overnight cultures of pGAPzα C from plate 3; transformed E. coli cells with pGAPzα C and plated.
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5/15 Group B: Restriction digest Group C: Made eight hour cultures from plates made 5/13; made YPDS plates (no antibiotics); plated P. pastoris on YPDS plates; made YPD broth; made overnight culture of P. pastoris in YPD from glycerol stock; made overnight culture of E. coli and pGAPzα C from eight hour culture.  
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Revision as of 21:29, 1 June 2013

Georgia State Wiki

5/15 Group B: Restriction digest Group C: Made eight hour cultures from plates made 5/13; made YPDS plates (no antibiotics); plated P. pastoris on YPDS plates; made YPD broth; made overnight culture of P. pastoris in YPD from glycerol stock; made overnight culture of E. coli and pGAPzα C from eight hour culture.