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Team:DTU-Denmark/Notebook/28 August 2013
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| - | A gradient PCR was made for pSB1C3 and pZA21::ara for Nir | + | A gradient PCR was made for pSB1C3 and pZA21::ara for Nir. |
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The [[Team:DTU-Denmark/Methods/PCR-mix|master mix]] contains: | The [[Team:DTU-Denmark/Methods/PCR-mix|master mix]] contains: | ||
*CG buffer, | *CG buffer, | ||
Revision as of 14:27, 29 August 2013
28 August 2013
Contents |
Lab 208
Main purpose
Who was in the lab
Henrike, Kristian, Julia
Procedure
A gradient PCR was made for pSB1C3 and pZA21::ara for Nir.
The master mix contains:
- CG buffer,
- X7 polymerase,
- 2% of DMSO (1uL of 100%DMSO) per reaction
- 1mM MgCl2 (1uL of 50mM MgCl2) per reaction
| 1 | 2 | |
|---|---|---|
| Template | pSB1C3 | pZA21::ara |
| Primers | 57a + 57b | 58a + 58b |
| Temperature range | 58C-78C | 50C-65C |
| Annealing time per cycle | 1:30 min | 2 min |
Each reaction was run in one of the 12 different annealing temperatures within the range indicated in the table.
Results
Conclusion
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