Team:Groningen/Labwork/29 August 2013

From 2013.igem.org

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The ligation reaction containing S15+S4+pSB1C3 and the positive control (only pSB1C3) were transformed in <i>E. Coli</i> DH5&alpha;.
The ligation reaction containing S15+S4+pSB1C3 and the positive control (only pSB1C3) were transformed in <i>E. Coli</i> DH5&alpha;.
<br>The competent cells were plated on LB + Cm and incubated at 37&deg;C overnight.
<br>The competent cells were plated on LB + Cm and incubated at 37&deg;C overnight.
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<h2>Sander</h2>
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made a plate with liquid SGG medium and a plastic object partially submerged and placed in a 30 C stove.
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Revision as of 14:31, 29 August 2013

Inne


09:15 plates were taken out of incubators.
10:12 the plates were put back into incubators.
16:25 plates taken out of incubators.

Claudio

The ligation reaction containing S15+S4+pSB1C3 and the positive control (only pSB1C3) were transformed in E. Coli DH5α.
The competent cells were plated on LB + Cm and incubated at 37°C overnight.

Sander

made a plate with liquid SGG medium and a plastic object partially submerged and placed in a 30 C stove.