Team:DTU-Denmark/Notebook/30 August 2013

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(Difference between revisions)
(Gel)
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* purification of pZA21::ara(tight) with endings for Nir
* purification of pZA21::ara(tight) with endings for Nir
* 1 kb ladder
* 1 kb ladder
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 +
[[File:2013-08-30 psbnir puri verification2.jpg|600px]]
==Conclusion==
==Conclusion==

Revision as of 17:27, 30 August 2013

30 August 2013

Contents

lab 208


Main purpose


Who was in the lab


Procedure


Results


Gel

  • 1 kb ladder
  • psB1C3 for Nir, 2% DMSO
  • psB1C3 for Nir, 2% DMSO, 1 uL 50mM MgCl2
  • psB1C3 for Nir, 4% DMSO
  • psB1C3 for Nir, 4% DMSO, 1 uL 50mM MgCl2
  • psB1C3 for Nir, 1M Betaine
  • purification of psB1C3 USER fragment to make Biobricks (escaped from well -> Ethanol?)
  • purification of HAO fragment (escaped from well -> Ethanol?)
  • purification of AMO fragment (escaped from well -> Ethanol?)
  • purification of psB1C3 USER fragment to make Biobricks (used more loading buffer)
  • purification of HAO fragment (used more loading buffer)
  • purification of AMO fragment (used more loading buffer)
  • purification of pZA21::ara(tight)
  • purification of pZA21::ara(tight) with endings for Nir
  • 1 kb ladder

2013-08-30 psbnir puri verification2.jpg

Conclusion


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