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From 2013.igem.org

Contents 1 Transformation results 2 Gel Electrophoresis 3 Gel purification of the pSB1C3 backbone and RFP biobrick 4 Double digest of TOD gene ligations

Transformation results

Gel Electrophoresis

• The double digest (pSB1C3) from yesterday was run on a 1% gel.
• This was done in order to confirm that the digest worked and also to purify the pSB1C3 vector.

• From left to right

1. Sample 1.1 - track 2
2. Sample 1.2 - track 3
3. Sample 2.1 - track 4
4. Sample 2.2 - track 5

• • The 2 Kb bands are the pSB1C3 backbone
  • The 1 Kb bands are the RFP biobrick

The two higher bands in tracks 2 and 3 are plasmids that were not digested.

Gel purification of the pSB1C3 backbone and RFP biobrick

• The Zymoclean Gel DNA recovery Kit was used to perform the purification and its protocol was followed.
• However the elution step was changed to 12ul of elution buffer, and this step was repeated.

Double digest of TOD gene ligations